caa a22 4500 477132383 CHVBK 20180405111707.0 cr unu---uuuuu 170330e19970901xx s 000 0 eng 10.1023/A:1018391409426 doi (NATIONALLICENCE)springer-10.1023/A:1018391409426 Specific binding of inorganic mercury to Na- K-ATPase in rat liver plasma membrane and signal transduction [Elektronische Daten] [Shelley Bhattacharya, Shambhunath Bose, Banibrata Mukhopadhyay, Debapriya Sarkar, Debaprasad Das, Jaya Bandyopadhyay, Rakhi Bose, Chandana Majumdar, Shawli Mondal, Sutapa Sen] Specific binding of Hg to ouabain-sensitive Na-K-ATPase of rat liver plasma membrane was demonstrated with a K of 2.64¥10 and B of 1.6nmole mg protein. The binding of mercury to the enzyme also causes significant inhibition of the enzyme, which is greater than its ouabain sensitivity. In the cytosol Hg binding to reduced glutathione (GSH) is stimulated by GSH-S-transferase (GST), the activity of which was found to be significantly enhanced by 15mM Na and 10mM Hg. It is proposed that the transport of Hg2 inside the cell takes place by increased dissociation of Hg from the membrane due to greater avidity of Hg towards cytosolar GSH binding. The GSH-Hg complex enters the nucleus where it dissociates to bind the metal response element (MRE) of the metallothionein (MT) gene to induce MT transcription. Chapman and Hall, 1997 mercury nationallicence Na-K-ATPase nationallicence rat liver nationallicence signal transduction nationallicence Bhattacharya Shelley Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut Bose Shambhunath Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut Mukhopadhyay Banibrata Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut Sarkar Debapriya Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut Das Debaprasad Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut Bandyopadhyay Jaya Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut Bose Rakhi Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut Majumdar Chandana Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut Mondal Shawli Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut Sen Sutapa Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut Biometals Kluwer Academic Publishers 10/3(1997-09-01), 157-162 0966-0844 10:3<157 1997 10 10534 https://doi.org/10.1023/A:1018391409426 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018391409426 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bhattacharya Shelley Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut NATIONALLICENCE 700 1- Bose Shambhunath Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut NATIONALLICENCE 700 1- Mukhopadhyay Banibrata Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut NATIONALLICENCE 700 1- Sarkar Debapriya Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut NATIONALLICENCE 700 1- Das Debaprasad Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut NATIONALLICENCE 700 1- Bandyopadhyay Jaya Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut NATIONALLICENCE 700 1- Bose Rakhi Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut NATIONALLICENCE 700 1- Majumdar Chandana Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut NATIONALLICENCE 700 1- Mondal Shawli Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut NATIONALLICENCE 700 1- Sen Sutapa Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University, Santiniketan, India aut NATIONALLICENCE 773 0- Biometals Kluwer Academic Publishers 10/3(1997-09-01), 157-162 0966-0844 10:3<157 1997 10 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer