caa a22 4500 477132421 CHVBK 20180405111707.0 cr unu---uuuuu 170330e19970901xx s 000 0 eng 10.1023/A:1018351728081 doi (NATIONALLICENCE)springer-10.1023/A:1018351728081 Iron uptake and intracellular metal transfer in mycobacteria mediated by xenosiderophores [Elektronische Daten] [Berthold Matzanke, Rudolf Böhnke, Ute Möllmann, Rolf Reissbrodt, Volker Schünemann, Alfred Trautwein] Growth promotion was tested using M. smegmatis wild type strain, an exochelin-deficient mutant, and M. fortuitum employing a broad variety of xenosiderophores including hydroxamates, catecholates and a-hydroxy carboxylic acids. The experiments revealed that utilization of siderophore-bound iron is substrate specific suggesting high-affinity siderophore receptor and transport systems. Concentration-dependent uptake of a selected xenosiderophore (fericrocin) in M. smegmatis showed saturation kinetics and uptake was inhibited by respiratory poisons. In situ Mössbauer spectroscopy of ferricrocin uptake in M. smegmatis indicated rapid intracellular reductive removal of the metal excluding intracellular ferricrocin accumulation. The ultimate intracellular iron pool is represented by a compound (δ = 0.43 mm s, DE = 1.03 mm s) which has also been found in many other microorganisms and does not represent a bacterioferritin, cytochrome or iron-sulfur cluster. By contrast, iron uptake via citrate - a compound exhibiting a very low complex stability constant - involves ligand exchange with mycobactin. Mycobactin has merely a transient role. The ultimate storage compound is an E.coli-type bacterioferritin, in which over 90% of cellular iron is located. Chapman and Hall, 1997 EPR nationallicence iron transport nationallicence Mössbauer spectroscopy nationallicence mycobacterium nationallicence xenosiderophores nationallicence Matzanke Berthold Isotopenlabor, Technisch-Naturwissenschaftliche Fakultät, Medizinische Universität, Lübeck, Germany aut Böhnke Rudolf Mikrobiologie/Biotechnologie, Eberhard-Karls-Universität, Tübingen, Germany aut Möllmann Ute Naturstoff-Forschung (HKI), Hans-Knöll-Institut, Jena, Germany aut Reissbrodt Rolf Robert Koch Institut, Wernigerode, Germany aut Schünemann Volker Institut für Physik, Medizinische Universität, Lübeck, Germany aut Trautwein Alfred Institut für Physik, Medizinische Universität, Lübeck, Germany aut Biometals Kluwer Academic Publishers 10/3(1997-09-01), 193-203 0966-0844 10:3<193 1997 10 10534 https://doi.org/10.1023/A:1018351728081 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018351728081 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matzanke Berthold Isotopenlabor, Technisch-Naturwissenschaftliche Fakultät, Medizinische Universität, Lübeck, Germany aut NATIONALLICENCE 700 1- Böhnke Rudolf Mikrobiologie/Biotechnologie, Eberhard-Karls-Universität, Tübingen, Germany aut NATIONALLICENCE 700 1- Möllmann Ute Naturstoff-Forschung (HKI), Hans-Knöll-Institut, Jena, Germany aut NATIONALLICENCE 700 1- Reissbrodt Rolf Robert Koch Institut, Wernigerode, Germany aut NATIONALLICENCE 700 1- Schünemann Volker Institut für Physik, Medizinische Universität, Lübeck, Germany aut NATIONALLICENCE 700 1- Trautwein Alfred Institut für Physik, Medizinische Universität, Lübeck, Germany aut NATIONALLICENCE 773 0- Biometals Kluwer Academic Publishers 10/3(1997-09-01), 193-203 0966-0844 10:3<193 1997 10 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer