caa a22 4500 47713243X CHVBK 20180405111707.0 cr unu---uuuuu 170330e19970901xx s 000 0 eng 10.1023/A:1018347627172 doi (NATIONALLICENCE)springer-10.1023/A:1018347627172 Characterization of a cobalt-resistant mutant of Neurospora crassa with transport block [Elektronische Daten] [L.S. Sajani, P. Maruthi Mohan] A cobalt-resistant strain of Neurospora crassa (cor) was obtained by repeated subculturing of the wild type on cobalt-containing agar medium. N. crassa cor is twentyfold more resistant to cobalt ions compared with the wild type. Resistance was stable on repeated subculturing of cor on cobalt-free media. N. crassa cor is also cross-resistant to nickel (fourfold), but not to zinc or copper. Higher concentrations of iron and magnesium ions are required to reverse growth inhibition due to cobalt toxicity in N. crassa cor, compared with the wild type. Germinating conidia and mycelia of the cor strain accumulated lower levels of cobalt ions compared with the parent N. crassa. The partial transport block for cobalt uptake is shown to be primarily due to decreased surface binding of cobalt to mycelia and cell walls. Efflux of mycelial cobalt was also observed in wild type and cobalt-resistant N. crassa. The characteristics of cor in comparison with wild type N. crassa are discussed in relation to the mechanisms of cobalt resistance. Chapman and Hall, 1997 cobalt binding nationallicence cobalt-resistant nationallicence cobalt uptake nationallicence metal-resistant nationallicence N. crassa nationallicence Sajani L.S. Department of Biochemistry, Osmania University, Hyderabad, India aut Maruthi Mohan P. Department of Biochemistry, Osmania University, Hyderabad, India aut Biometals Kluwer Academic Publishers 10/3(1997-09-01), 175-183 0966-0844 10:3<175 1997 10 10534 https://doi.org/10.1023/A:1018347627172 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018347627172 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sajani L.S. Department of Biochemistry, Osmania University, Hyderabad, India aut NATIONALLICENCE 700 1- Maruthi Mohan P. Department of Biochemistry, Osmania University, Hyderabad, India aut NATIONALLICENCE 773 0- Biometals Kluwer Academic Publishers 10/3(1997-09-01), 175-183 0966-0844 10:3<175 1997 10 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer