caa a22 4500 477132480 CHVBK 20180405111707.0 cr unu---uuuuu 170330e19971201xx s 000 0 eng 10.1023/A:1018316214877 doi (NATIONALLICENCE)springer-10.1023/A:1018316214877 Effects of Pb2+, Ni2+, Hg2+ and Se4+ on cultured cells. Analysis of uptake, toxicity and influence on radiosensitivity [Elektronische Daten] [Peter Frisk, Anders Saetre, Beatriz Couce, Bo Stenerlöw, Jörgen Carlsson, Ulf Lindh] Effects of Pb2+, Ni2+, Hg2+ and Se4+ on cultured human glioma U-343MG cells were investigated considering uptake, toxicity and, in combination with radiation, clonogenic cell survival. The cells were exposed to 0-100 μm of the metals for a week before the evaluation. The tests showed a tendency to toxicity with 10 μm nickel although not significant (P > 0.05). Selenium, lead and mercury exerted a significant toxicity (P < 0.05) at 2.5 μm, 10 μm and 1 μm, respectively. To challenge the clonogenic cell survival capacity, the cells were irradiated with60Co photons after being exposed to the highest nontoxic concentration of the different metals. The clonogenic cell survival tests, after irradiation, showed no significant change if the cells were exposed to 5 μm nickel, 0.5 μm selenium or 5 μm lead compared with those not exposed. Mercury, 0.1 μm, gave a relative reduction in survival compared with only irradiated cells of 58 ± 17%. Thus, only mercury affected the radiation-induced damage and/or repair. When exposed to the highest nontoxic concentrations of the different metals, the cultures did not display a significant uptake ratio (metal concentration ratio of exposed cells to control cells) of nickel (3.1 ± 3.3), only a small uptake ratio of selenium (4.0 ± 0.4), while there was a large uptake ratio of both lead (2.6 ± 1.7) x 102 and mercury (1.5 ± 0.2) x 101. The results indicated that nickel was neither especially toxic nor influenced the clonogenic cell survival after irradiation. Mercury was more toxic and also influenced the radiation sensitivity. Lead was taken up strongly but did not influence the radiation sensitivity. Selenium accumulated but gave no detectable effect on the radiation sensitivity. Chapman and Hall, 1997 lead nationallicence mercury nationallicence metals nationallicence nickel nationallicence radiation effects nationallicence selenium nationallicence Frisk Peter Division of Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden aut Saetre Anders Division of Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden aut Couce Beatriz Division of Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden aut Stenerlöw Bo Division of Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden aut Carlsson Jörgen Division of Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden aut Lindh Ulf Division of Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden aut Biometals Kluwer Academic Publishers 10/4(1997-12-01), 263-270 0966-0844 10:4<263 1997 10 10534 https://doi.org/10.1023/A:1018316214877 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018316214877 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Frisk Peter Division of Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden aut NATIONALLICENCE 700 1- Saetre Anders Division of Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden aut NATIONALLICENCE 700 1- Couce Beatriz Division of Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden aut NATIONALLICENCE 700 1- Stenerlöw Bo Division of Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden aut NATIONALLICENCE 700 1- Carlsson Jörgen Division of Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden aut NATIONALLICENCE 700 1- Lindh Ulf Division of Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden aut NATIONALLICENCE 773 0- Biometals Kluwer Academic Publishers 10/4(1997-12-01), 263-270 0966-0844 10:4<263 1997 10 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer