caa a22 4500 477132502 CHVBK 20180405111707.0 cr unu---uuuuu 170330e19971201xx s 000 0 eng 10.1023/A:1018364130807 doi (NATIONALLICENCE)springer-10.1023/A:1018364130807 Purification of a fully metal-depleted Cu, Zn superoxide dismutase from copper-deficient rat liver [Elektronische Daten] [Luisa Rossi, Eliana Marchese, Angelo De Martino, Giuseppe Rotilio, Maria Ciriolo] A copper-deprived form of the enzyme Cu, Zn superoxide dismutase was identifiedin the liver of rats made copper-deficient by dietary restriction. In homogenates ofsuch livers Cu, Zn superoxide dismutase presents a dis-homogeneous electrophoreticprofile with respect to the native enzyme. When rat liver extracts were treated withexogenous copper an electrophoretic pattern resembling the native one was observed.Enzyme purified by chromatography on DE-52 resin shows two major components, onecorresponding to genuine, native enzyme and another one, eluting at higher ionicstrength. The latter protein (Fraction II) consists of several isoforms which showthe same characteristics of the native superoxide dismutase as far as immunoreactivityand molecular weight are concerned, but with decreased contents of copper and zinc. Itscatalytic constant, referring to copper content, was 15 times lower than that obtainedfor the native enzyme. Moreover, the catalytic power of purified Fraction II was notregained upon incubation with copper. The occurrence of a superoxide dismutase voidof metals confirms the hypothesis that this protein plays a dual physiological role:in metal metabolism and in superoxide anion dismutation. Chapman and Hall, 1997 copper-deficiency nationallicence rat liver nationallicence superoxide dismutase nationallicence purification nationallicence Rossi Luisa Department of Biology, University of Rome ‘Tor Vergata', Rome aut Marchese Eliana Department of Biology, University of Rome ‘Tor Vergata', Rome aut De Martino Angelo Department of Biology, University of Rome ‘Tor Vergata', Rome aut Rotilio Giuseppe Department of Biology, University of Rome ‘Tor Vergata', Rome aut Ciriolo Maria Institute of Biological Sciences, University of Chieti ‘G. D'Annunzio', Chieti, Italy aut Biometals Kluwer Academic Publishers 10/4(1997-12-01), 257-262 0966-0844 10:4<257 1997 10 10534 https://doi.org/10.1023/A:1018364130807 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018364130807 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rossi Luisa Department of Biology, University of Rome ‘Tor Vergata', Rome aut NATIONALLICENCE 700 1- Marchese Eliana Department of Biology, University of Rome ‘Tor Vergata', Rome aut NATIONALLICENCE 700 1- De Martino Angelo Department of Biology, University of Rome ‘Tor Vergata', Rome aut NATIONALLICENCE 700 1- Rotilio Giuseppe Department of Biology, University of Rome ‘Tor Vergata', Rome aut NATIONALLICENCE 700 1- Ciriolo Maria Institute of Biological Sciences, University of Chieti ‘G. D'Annunzio', Chieti, Italy aut NATIONALLICENCE 773 0- Biometals Kluwer Academic Publishers 10/4(1997-12-01), 257-262 0966-0844 10:4<257 1997 10 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer