caa a22 4500 477132618 CHVBK 20180405111708.0 cr unu---uuuuu 170330e19971201xx s 000 0 eng 10.1023/A:1018360029898 doi (NATIONALLICENCE)springer-10.1023/A:1018360029898 Reduction of vanadate to vanadyl by a strain of Saccharomyces cerevisiae [Elektronische Daten] [Laura Bisconti, Milva Pepi1, Stefano Mangani, Franco Baldi] Three strains of Saccharomyces cerevisiae, SC-1, DBVPG 6173 and DBVPG 6037, were studied for vanadate resistance in complex Sabouraud medium since they did not thrive in different minimal media (yeast nitrogen base with and without amino acids). The strain SC-1 was resistant up to 16 mm of vanadate, whereas the strains DBVPG 6173 and DBVPG 6037 were inhibited by 8 mm and 4 mm vanadate, respectively. The vanadate resistance in strain SC-1 was constitutive and due to the reduction of this oxyanion to vanadyl, which was detected by EPR spectroscopy and visible spectroscopy. The transformation of vanadate to vanadyl took place during the exponential growth phase; 10 mm of vanadate was reduced to vanadyl outside the cells since the oxyanion was not detected in the cell biomass and only a negligible concentration of vanadyl (25 nmoles mg cells dry weight) was found in the biomass. The other two vanadate-sensitive yeast strains only accumulated vanadate and did not reduce the oxyanion to vanadyl. Chapman and Hall, 1997 NMR and EPR spectroscopy nationallicence speciation nationallicence transformation nationallicence vanadate nationallicence vanadyl nationallicence yeast nationallicence Bisconti Laura Dipartimento di Biologia, Ambientale aut Pepi1 Milva Dipartimento di Biologia, Ambientale aut Mangani Stefano Dipartimento di Chimica, Università di Siena, Siena, Italy aut Baldi Franco Dipartimento di Biologia, Ambientale aut Biometals Kluwer Academic Publishers 10/4(1997-12-01), 239-246 0966-0844 10:4<239 1997 10 10534 https://doi.org/10.1023/A:1018360029898 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018360029898 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bisconti Laura Dipartimento di Biologia, Ambientale aut NATIONALLICENCE 700 1- Pepi1 Milva Dipartimento di Biologia, Ambientale aut NATIONALLICENCE 700 1- Mangani Stefano Dipartimento di Chimica, Università di Siena, Siena, Italy aut NATIONALLICENCE 700 1- Baldi Franco Dipartimento di Biologia, Ambientale aut NATIONALLICENCE 773 0- Biometals Kluwer Academic Publishers 10/4(1997-12-01), 239-246 0966-0844 10:4<239 1997 10 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer