caa a22 4500 477132634 CHVBK 20180405111708.0 cr unu---uuuuu 170330e19970601xx s 000 0 eng 10.1023/A:1018335324447 doi (NATIONALLICENCE)springer-10.1023/A:1018335324447 Proliferative and morphological changes induced by vanadium compounds on Swiss 3T3 fibroblasts [Elektronische Daten] [Ana Cortizo, Viviana Sa´lice, Cecilia Vescina, Susana Etcheverry] Vanadium compounds are shown to have a mitogenic effect on fibroblast cells. The effects of vanadate, vanadyl and pervanadate on the proliferation and morphological changes of Swiss 3T3 cells in culture are compared. Vanadium derivatives induced cell proliferation in a biphasic manner, with a toxic-like effect at doses over 50mM, after 24h of incubation. Vanadyl and vanadate were equally potent at 2.5-10mM. At 50mM vanadate inhibited cell proliferation, whereas slight inhibition was observed at 100mM of vanadyl. At 10mM pervanadate was as potent as vanadate and vanadyl in stimulating fibroblast proliferation, but no effect was observed at lower concentrations. A pronounced cytotoxic-like effect was induced by pervanadate at 50mM. All of these effects were accompanied by morphological changes: transformation of fibroblast shape from polygonal to fusiform; retraction with cytoplasm condensation; and loss of lamellar processes. The magnitude of these transformations correlates with the potency of vanadium derivatives to induce a cytotoxic-like effect: pervanadate>vanadate>vanadyl. These data suggest that the oxidation state and coordination geometry of vanadium determine the degree of the cytotoxicity. Chapman and Hall, 1997 cytotoxicity nationallicence fibroblasts nationallicence growth factors nationallicence proliferation nationallicence vanadium nationallicence Cortizo Ana Ca´tedra de Bioquímica Patolo´gica, Patolo´gica, Argentina aut Sa´lice Viviana Ca´tedra de Bioquímica Patolo´gica, Patolo´gica, Argentina aut Vescina Cecilia Ca´tedra de Bioquímica Patolo´gica, Patolo´gica, Argentina aut Etcheverry Susana CEQUINOR, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina aut Biometals Kluwer Academic Publishers 10/2(1997-06-01), 127-133 0966-0844 10:2<127 1997 10 10534 https://doi.org/10.1023/A:1018335324447 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018335324447 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cortizo Ana Ca´tedra de Bioquímica Patolo´gica, Patolo´gica, Argentina aut NATIONALLICENCE 700 1- Sa´lice Viviana Ca´tedra de Bioquímica Patolo´gica, Patolo´gica, Argentina aut NATIONALLICENCE 700 1- Vescina Cecilia Ca´tedra de Bioquímica Patolo´gica, Patolo´gica, Argentina aut NATIONALLICENCE 700 1- Etcheverry Susana CEQUINOR, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina aut NATIONALLICENCE 773 0- Biometals Kluwer Academic Publishers 10/2(1997-06-01), 127-133 0966-0844 10:2<127 1997 10 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer