caa a22 4500 477132650 CHVBK 20180405111708.0 cr unu---uuuuu 170330e19970601xx s 000 0 eng 10.1023/A:1018370804882 doi (NATIONALLICENCE)springer-10.1023/A:1018370804882 Iron supplementation moderates but does not cure the Belgrade anemia [Elektronische Daten] [Michael Garrick, Donna Scott, Susannah Walpole, Eric Finkelstein, Joy Whitbred, Sandeep Chopra, Lalitha Trivikram, David Mayes, Daphne Rhodes, Kimberly Cabbagestalk, Rahmi Oklu, Adnan Sadiq, Brett Mascia, James Hoke, Laura Garrick] Belgrade rats inherit microcytic, hypochromic anemia as an autosomalrecessive trait (gene symbol b). Erythrocytes and tissue are iron deficientin the face of elevated TIBC (total iron binding capacity) and percent ironsaturation; iron injections increased the number of erythrocytes but theirappearance remained abnormal. We have investigated iron supplements toimprove husbandry of b/b rats and to learn more about the underlying defectand its tissue distribution. Weekly IM (intramuscular) injections ofiron-dextran (Imferon at 30 mg kg) improved the anemia but did not alter thered cell morphology. Certain diets also improved the health of b/b rats whencompared to standard rat chows by the criteria of weight, survival toadulthood, hematology and reproduction. The critical nutritional factorturned out to be iron bioavailability, with ferrous iron added to the dietimproving the health of Belgrade rats without affecting the underlyingerythroid defect. Tissue iron measurements after dietary or parenteralsupplementation confirmed the iron deficient status of untreated b/b rats andestablished that dietary ferrous iron partially relieved this deficiency,with injections leading to greater amounts of tissue iron. Serum iron andTIBC were also found to be elevated in untreated b/b rats, with dietarysupplementation decreasing but not eliminating the elevation in TIBC. Thesestudies indicate that iron supplements can improve the health of b/b ratswithout altering the underlying defect and also suggest that the mutationcould alter iron uptake in the GI (gastrointestinal) tract. Chapman and Hall, 1997 Belgrade rat nationallicence dietary iron nationallicence ferrous nationallicence parenteral iron nationallicence serum iron nationallicence Garrick Michael Department of Biochemistry, SUNY, Buffalo, NY, USA aut Scott Donna Department of Biochemistry, SUNY, Buffalo, NY, USA aut Walpole Susannah Department of Biochemistry, SUNY, Buffalo, NY, USA aut Finkelstein Eric Department of Biochemistry, SUNY, Buffalo, NY, USA aut Whitbred Joy Department of Biochemistry, SUNY, Buffalo, NY, USA aut Chopra Sandeep Department of Biochemistry, SUNY, Buffalo, NY, USA aut Trivikram Lalitha Department of Biochemistry, SUNY, Buffalo, NY, USA aut Mayes David Department of Biochemistry, SUNY, Buffalo, NY, USA aut Rhodes Daphne Department of Biochemistry, SUNY, Buffalo, NY, USA aut Cabbagestalk Kimberly Department of Biochemistry, SUNY, Buffalo, NY, USA aut Oklu Rahmi Department of Biochemistry, SUNY, Buffalo, NY, USA aut Sadiq Adnan Department of Biochemistry, SUNY, Buffalo, NY, USA aut Mascia Brett Department of Biochemistry, SUNY, Buffalo, NY, USA aut Hoke James Department of Biochemistry, SUNY, Buffalo, NY, USA aut Garrick Laura Department of Biochemistry, SUNY, Buffalo, NY, USA aut Biometals Kluwer Academic Publishers 10/2(1997-06-01), 65-76 0966-0844 10:2<65 1997 10 10534 https://doi.org/10.1023/A:1018370804882 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018370804882 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Garrick Michael Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Scott Donna Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Walpole Susannah Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Finkelstein Eric Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Whitbred Joy Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Chopra Sandeep Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Trivikram Lalitha Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Mayes David Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Rhodes Daphne Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Cabbagestalk Kimberly Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Oklu Rahmi Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Sadiq Adnan Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Mascia Brett Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Hoke James Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Garrick Laura Department of Biochemistry, SUNY, Buffalo, NY, USA aut NATIONALLICENCE 773 0- Biometals Kluwer Academic Publishers 10/2(1997-06-01), 65-76 0966-0844 10:2<65 1997 10 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer