caa a22 4500 477132693 CHVBK 20180405111708.0 cr unu---uuuuu 170330e19970601xx s 000 0 eng 10.1023/A:1018379106700 doi (NATIONALLICENCE)springer-10.1023/A:1018379106700 Metabolism-independent binding of toxic metals by Ulva lactuca: cadmium binds to oxygen-containing groups, as determined by NMR [Elektronische Daten] [Elizabeth Webster, Andrew Murphy, John Chudek, Geoffrey Gadd] The metabolism-independent metal binding characteristics of Ulva lactuca were investigated using both freeze-dried thalli and cell walls stripped of intracellular material by incubation in Triton-X followed by methanol. Biosorption of Cd, Zn, Cu and Co by freeze-dried thallus was concentration-dependent and followed Freundlich and Langmuir isotherms. The Freundlich plot suggested that freeze-dried U. lactuca had the greatest binding affinity for Cu compared with Cd, Zn and Co. The BET (Brunauer-Emmett-Teller) plot, which indicates a more complex form of adsorption, and the Scatchard plot were not adequate models for Cu adsorption. The Scatchard plot of Cd suggested that two Cd binding sites were available on the freeze-dried thallus, with the second, lower affinity site only becoming available at Cd loading capacities greater than 4.9mmol g dry wt. Cd nuclear magnetic resonance (NMR) studies confirmed that two binding sites were available for Cd on the freeze-dried algal powder, though only one was available on the cell wall, and that the affinity of the binding sites was greater for Cu than for Cd. The results of the NMR experiments suggested that Cd binds to oxygen-containing functional groups in the algal powder and on the cell wall. It is proposed that sulphate or hydroxyl groups attached to polysaccharide subunits are possible sites. Chapman and Hall, 1997 BET nationallicence biosorption nationallicence cadmium nationallicence cobalt nationallicence copper nationallicence Freundlich nationallicence NMR nationallicence Scatchard nationallicence Ulva lactuca nationallicence zinc nationallicence Webster Elizabeth Department of Biological Sciences, University of Dundee, Dundee, UK aut Murphy Andrew Department of Chemistry, University of Dundee, Dundee, UK aut Chudek John Department of Chemistry, University of Dundee, Dundee, UK aut Gadd Geoffrey Department of Biological Sciences, University of Dundee, Dundee, UK aut Biometals Kluwer Academic Publishers 10/2(1997-06-01), 105-117 0966-0844 10:2<105 1997 10 10534 https://doi.org/10.1023/A:1018379106700 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018379106700 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Webster Elizabeth Department of Biological Sciences, University of Dundee, Dundee, UK aut NATIONALLICENCE 700 1- Murphy Andrew Department of Chemistry, University of Dundee, Dundee, UK aut NATIONALLICENCE 700 1- Chudek John Department of Chemistry, University of Dundee, Dundee, UK aut NATIONALLICENCE 700 1- Gadd Geoffrey Department of Biological Sciences, University of Dundee, Dundee, UK aut NATIONALLICENCE 773 0- Biometals Kluwer Academic Publishers 10/2(1997-06-01), 105-117 0966-0844 10:2<105 1997 10 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer