caa a22 4500 477133053 CHVBK 20180405111709.0 cr unu---uuuuu 170330e19970301xx s 000 0 eng 10.1023/A:1002911522748 doi (NATIONALLICENCE)springer-10.1023/A:1002911522748 Chromosome doubling and fertility study of Alstroemeria aurea × A. caryophyllaea [Elektronische Daten] [Chunsheng Lu, Mark Bridgen] Self-pollinations of a diploid (2n = 2x = 16) interspecific hybrid Alstroemeria aurea × A. caryophyllaea, resulted in no seeds. Backcrosses of the hybrid with parent A. aurea did not produce any seeds. In an attempt to restore the hybrid fertility, an efficient in vitro procedure has been developed and applied effectively in the chromosome doubling of the diploid hybrid. Forty-one percent of the treated plants were proven to be truly tetraploid by chromosome counts and stomatal measurements after applying 0.2 to 0.6% colchicine for 6 to 24 hours. Over 87.5% of these colchicine-induced tetraploids were stable and retained their tetraploidy after one year of growth. The fertility of the hybrid was not restored although the pollen stainability was increased from 0 to 12% after chromosome doubling. Cytological studies on the pollen mother cells (PMCs) of the sterile diploid hybrids revealed abnormal meiotic behaviors. In addition, aneuploid chromosome numbers, ranging from 2n = 1 to 2n = 18, were observed in over 45% of the PMCs examined. PMCs of the colchicine-induced tetraploids showed that meiotic chromosome pairings were normal in most cases (1.59I + 15.07II + 0.05III + 0.03IV). These results indicate that the sterility of this hybrid is not only caused by parental chromosome differences, but other complex fertility/sterility-regulating mechanisms are involved too. Kluwer Academic Publishers, 1997 colchicine nationallicence Alstroemeria sp nationallicence alstroemeria nationallicence Inca Lily nationallicence micronucleus nationallicence ovule culture nationallicence polyploid nationallicence sterility genes nationallicence Lu Chunsheng Department of Plant Science, U-67, University of Connecticut, 06269, Storrs, CT, USA aut Bridgen Mark Department of Plant Science, U-67, University of Connecticut, 06269, Storrs, CT, USA aut Euphytica Kluwer Academic Publishers 94/1(1997-03-01), 75-81 0014-2336 94:1<75 1997 94 10681 https://doi.org/10.1023/A:1002911522748 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002911522748 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Chunsheng Department of Plant Science, U-67, University of Connecticut, 06269, Storrs, CT, USA aut NATIONALLICENCE 700 1- Bridgen Mark Department of Plant Science, U-67, University of Connecticut, 06269, Storrs, CT, USA aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 94/1(1997-03-01), 75-81 0014-2336 94:1<75 1997 94 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer