caa a22 4500 47713310X CHVBK 20180405111709.0 cr unu---uuuuu 170330e19970301xx s 000 0 eng 10.1023/A:1002962724080 doi (NATIONALLICENCE)springer-10.1023/A:1002962724080 Plant regeneration from protoplasts isolated from primary calli using mature embryos of two Brazilian rice cultivars [Elektronische Daten] [Daniel Moura, Francisco Zapata-Arias, Akihiko Ando, Augusto Tulmann Neto] A plant regeneration system from rice protoplasts using calli derived from mature embryos was established for the two Brazilian modern rice cultivars IAC-201 and IAC-165. After 30 to 40 days of in vitro culture it was possible to obtain on average 6 million protoplasts per gram of callus. Microscopic selection of embryogenic calli was a key step for protoplast isolation. The production of embryogenic calli increased when L-proline and casein hydrolysate were used in the callus induction medium. The Oc or IR52 nurse cell lines were essential for protoplast division. Different regeneration media were studied and 139 plants were regenerated which set seed. Some of the regenerated plants showed morphological variation such as the presence of awns in spite of the short time of the in vitro culture. Kluwer Academic Publishers, 1997 Oryza sativa nationallicence japonica rice cultivar nationallicence mature embryo nationallicence primary calli nationallicence protoplasts nationallicence green plant regeneration nationallicence Moura Daniel Departmento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz', Universidade de Sao Paulo, Piracicaba, Sao Paulo, Brasil aut Zapata-Arias Francisco Departmento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz', Universidade de Sao Paulo, Piracicaba, Sao Paulo, Brasil aut Ando Akihiko Departmento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz', Universidade de Sao Paulo, Piracicaba, Sao Paulo, Brasil aut Tulmann Neto Augusto Seçao de Radiogenética, Centro de Energia Nuclear na Agricultura (CENA), Universidade de Sao Paulo, Piracicaba, Sao Paulo, Brasil aut Euphytica Kluwer Academic Publishers 94/1(1997-03-01), 01-05 0014-2336 94:1<01 1997 94 10681 https://doi.org/10.1023/A:1002962724080 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002962724080 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moura Daniel Departmento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz', Universidade de Sao Paulo, Piracicaba, Sao Paulo, Brasil aut NATIONALLICENCE 700 1- Zapata-Arias Francisco Departmento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz', Universidade de Sao Paulo, Piracicaba, Sao Paulo, Brasil aut NATIONALLICENCE 700 1- Ando Akihiko Departmento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz', Universidade de Sao Paulo, Piracicaba, Sao Paulo, Brasil aut NATIONALLICENCE 700 1- Tulmann Neto Augusto Seçao de Radiogenética, Centro de Energia Nuclear na Agricultura (CENA), Universidade de Sao Paulo, Piracicaba, Sao Paulo, Brasil aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 94/1(1997-03-01), 01-05 0014-2336 94:1<01 1997 94 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer