caa a22 4500 477133118 CHVBK 20180405111709.0 cr unu---uuuuu 170330e19970301xx s 000 0 eng 10.1023/A:1002967607727 doi (NATIONALLICENCE)springer-10.1023/A:1002967607727 Transgenic sugar beet tolerant to glyphosate [Elektronische Daten] [Marie Mannerlöf, Stig Tuvesson, Per Steen, Paul Tenning] Sugar beet (Beta vulgaris L.) lines transformed with the 5-enolpyruvylshikimate-3-phosphate synthase gene (CP4 EPSPS) from Agrobacterium sp. CP4 and a glyphosate oxidase reductase gene (GOX) also isolated from bacteria resulted in the development of lines highly tolerant to glyphosate. Glyphosate (N-phosphonomethyl-glycine) is the active ingredient in Roundup®, herbicide. The EPSPS enzyme is involved in the biosynthesis of aromatic amino acids. Glyphosate binds irreversible to the EPSPS and inhibits the pathway. GOX degrades glyphosate into non-toxic compounds. 260 independent transformants have been evaluated in greenhouse and field trials for tolerance to Roundup® in 1993 and 1994. Variation of tolerance was recorded between different transformants, ranging from complete susceptibility to full tolerance. The Agrobacterium tumefaciens mediated transformation resulted in a negative correlation between copy number of the T-DNA insert and the level of tolerance to the herbicide. Transformants which contain a single copy insert showed tolerance to higher doses of glyphosate than transformants with multiple copies. Two transgenic lines were identified that showed agronomically useful tolerance to glyphosate. Kluwer Academic Publishers, 1997 Agrobacterium tumefaciens nationallicence Beta vulgaris nationallicence 5-enolpyruvylshikimate-3-synthase nationallicence glyphosate nationallicence herbicide nationallicence sugar beet nationallicence transformation nationallicence Mannerlöf Marie HilleshöAB, 302, S-261 23, Landskrona, Sweden aut Tuvesson Stig HilleshöAB, 302, S-261 23, Landskrona, Sweden aut Steen Per Danisco Seed, 29, Dk-4960, Holeby, Denmark aut Tenning Paul HilleshöAB, 302, S-261 23, Landskrona, Sweden aut Euphytica Kluwer Academic Publishers 94/1(1997-03-01), 83-91 0014-2336 94:1<83 1997 94 10681 https://doi.org/10.1023/A:1002967607727 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002967607727 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mannerlöf Marie HilleshöAB, 302, S-261 23, Landskrona, Sweden aut NATIONALLICENCE 700 1- Tuvesson Stig HilleshöAB, 302, S-261 23, Landskrona, Sweden aut NATIONALLICENCE 700 1- Steen Per Danisco Seed, 29, Dk-4960, Holeby, Denmark aut NATIONALLICENCE 700 1- Tenning Paul HilleshöAB, 302, S-261 23, Landskrona, Sweden aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 94/1(1997-03-01), 83-91 0014-2336 94:1<83 1997 94 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer