caa a22 4500 477133231 CHVBK 20180405111710.0 cr unu---uuuuu 170330e19970401xx s 000 0 eng 10.1023/A:1002953929890 doi (NATIONALLICENCE)springer-10.1023/A:1002953929890 Induction and development of adventitious shoots of Atropa baetica as a means of propagation [Elektronische Daten] [Rafael Zarate, Manuel Cantos, Antonio Troncoso] In vitro propagation of Atropa baetica was established employing axillary buds. Single buds were cultured through a multiple shoot induction phase, rooting phase, and then followed by acclimatization in soil. For multiple shoot induction, Murashige and Skoog (MS) medium with 3% sucrose, supplemented with either 0.75 or 1.25 mg l-1 of BAP provided the best results with an average of 5.6 shoots per explant after 31 days of culture. Similar results were obtained with higher BAP concentrations (1.75-2.0 mg l-1); however, these media had a negative effect on the subsequent root induction due to residual BAP effect. Medium containing only 0.25 mg l-1 of BAP induced a significantly lower number of shoots. Root induction occurred spontaneously after transferring the shoots onto MS medium lacking any plant growth regulator. Moreover, root induction also occurred on media supplemented with 0.125 and 0.25 mg l-1 of NAA. On these two rooting media, this response was more prominent and with a higher number of roots per explant. Nevertheless, after 28 days on root induction medium, the number of rooted plantlets was similar on the three media. Acclimatization of plantlets in soil was very successful (95.52%). However, all plantlets which died during acclimatization were rooted on medium containing 0.25 mg l-1 NAA suggesting a negative carry over effect of this medium upon plantlet survival, irrespective of the initial BAP treatment used. On the other hand, karyological studies showed no variation in the number of chromosome (2n=72) in root tips of the plantlets produced. Kluwer Academic Publishers, 1997 Atropa baetica nationallicence micropropagation nationallicence species preservation nationallicence Zarate Rafael Consejo Superior de Investigationes Científicas (CSIC), IRNAS Sevilla, P.O. Box 1052, 41080, Sevilla, Spain aut Cantos Manuel Consejo Superior de Investigationes Científicas (CSIC), IRNAS Sevilla, P.O. Box 1052, 41080, Sevilla, Spain aut Troncoso Antonio Consejo Superior de Investigationes Científicas (CSIC), IRNAS Sevilla, P.O. Box 1052, 41080, Sevilla, Spain aut Euphytica Kluwer Academic Publishers 94/3(1997-04-01), 361-366 0014-2336 94:3<361 1997 94 10681 https://doi.org/10.1023/A:1002953929890 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002953929890 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zarate Rafael Consejo Superior de Investigationes Científicas (CSIC), IRNAS Sevilla, P.O. Box 1052, 41080, Sevilla, Spain aut NATIONALLICENCE 700 1- Cantos Manuel Consejo Superior de Investigationes Científicas (CSIC), IRNAS Sevilla, P.O. Box 1052, 41080, Sevilla, Spain aut NATIONALLICENCE 700 1- Troncoso Antonio Consejo Superior de Investigationes Científicas (CSIC), IRNAS Sevilla, P.O. Box 1052, 41080, Sevilla, Spain aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 94/3(1997-04-01), 361-366 0014-2336 94:3<361 1997 94 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer