caa a22 4500 477133266 CHVBK 20180405111710.0 cr unu---uuuuu 170330e19970401xx s 000 0 eng 10.1023/A:1002966309224 doi (NATIONALLICENCE)springer-10.1023/A:1002966309224 An assessment of somaclonal variation as a breeding tool for generating herbicide tolerant genotypes in wheat (Triticum aestivum L.) [Elektronische Daten] [R. Bozorgipour, J.W. Snape] The present work was initiated to assess tissue culture techniques as a means of generating and selecting herbicide tolerant genotypes of wheat through exploiting somaclonal variation. Callus was initiated from immature embryos of genetically defined varieties and subcultured onto selective media containing 5 μm, 10 μm and 50 μm concentrations of difenzoquat and atrazine. Plants were regenerated from all the selective media except that media containing the highest herbicide concentration. The progenies of the regenerated plants were tested as whole plants for their response to spray application of the herbicides. For difenzoquat, variation in response from extreme susceptibility to tolerance was observed. However, genetic characterization by progeny testing tolerant lines revealed that the induced variation was not heritable. No plants tolerant to atrazine were obtained. Overall, no clear evidence of heritable mutations was obtained. Alternative strategies to obtain herbicide tolerant genotypes using cell culture techniques are discussed. Kluwer Academic Publishers, 1997 somaclonal variation nationallicence in vitro selection nationallicence herbicides nationallicence wheat nationallicence triticum aestivum nationallicence Bozorgipour R. Seed and Plant Improvement Institute, Mardabad Avenue, 31585, Karaj, Iran aut Snape J.W. JI Centre, Colney Lane, NR4 7UJ, Norwich, U.K aut Euphytica Kluwer Academic Publishers 94/3(1997-04-01), 335-340 0014-2336 94:3<335 1997 94 10681 https://doi.org/10.1023/A:1002966309224 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002966309224 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bozorgipour R. Seed and Plant Improvement Institute, Mardabad Avenue, 31585, Karaj, Iran aut NATIONALLICENCE 700 1- Snape J.W. JI Centre, Colney Lane, NR4 7UJ, Norwich, U.K aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 94/3(1997-04-01), 335-340 0014-2336 94:3<335 1997 94 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer