caa a22 4500 477133398 CHVBK 20180405111710.0 cr unu---uuuuu 170330e19970301xx s 000 0 eng 10.1023/A:1002998121106 doi (NATIONALLICENCE)springer-10.1023/A:1002998121106 The use of a modified bulk segregant analysis to identify a molecular marker linked to a scab resistance gene in apple [Elektronische Daten] [Hong Yang, Schuyler Korban, Jutta Krüger, Hanna Schmidt] Almost two-hundred random sequence decamer-primers were used to screen a pair of bulked samples and the donor parent Malus floribunda clone 821 for markers linked to the Vf gene conferring resistance to apple scab (Venturia inaequalis (Cke.) Wint.). A single primer was identified which generated a PCR fragment, OPK16/1300, from the donor parent M. floribunda clone 821 and the scab-resistant selections/cultivars bulk, but not from the scab-susceptible recurrent parent bulk. Co-segregation analysis using a segregating apple progeny and polymorphism analysis of individual scab-resistant Coop selections/cultivars confirmed that this marker was linked to the scab-resistance gene Vf with a recombination frequency of 4.3%. OPK16/1300 was then cloned and sequenced. Sequence-specific primers of 25 oligonucleotides based on the marker were synthesized, and used in turn to screen M. floribunda clone 821, scab-susceptible apple cultivars, scab-resistant apple cultivars, and scab-resistant Coop selections. A pair of sequence-specific primers of clone OPK16/1300 amplified a distinct single band of the same size as the RAPD clone. Thus, a sequence characterized amplified region (SCAR) marker was developed which can be used to identify polymorphisms of OPK16/1300 based on the presence or absence of a single band. Kluwer Academic Publishers, 1997 Malus nationallicence apple nationallicence apple scab resistance nationallicence Venturia inaequalis nationallicence Vf gene nationallicence bulked segregant analysis nationallicence RAPDs nationallicence SCAR nationallicence Yang Hong Department of Natural Resources and Environmental Sciences, University of Illinois, 310 Madigan Building, 1201 W. Gregory Drive, 61801, Urbana, IL, U.S.A aut Korban Schuyler Department of Natural Resources and Environmental Sciences, University of Illinois, 310 Madigan Building, 1201 W. Gregory Drive, 61801, Urbana, IL, U.S.A aut Krüger Jutta Federal Centre for Breeding Research on Cultivated Plants, Institute for Ornamental Plant Breeding, Bornkampsweg 31, 22916, Ahrensburg, Germany aut Schmidt Hanna Federal Centre for Breeding Research on Cultivated Plants, Institute for Ornamental Plant Breeding, Bornkampsweg 31, 22916, Ahrensburg, Germany aut Euphytica Kluwer Academic Publishers 94/2(1997-03-01), 175-182 0014-2336 94:2<175 1997 94 10681 https://doi.org/10.1023/A:1002998121106 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002998121106 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Hong Department of Natural Resources and Environmental Sciences, University of Illinois, 310 Madigan Building, 1201 W. Gregory Drive, 61801, Urbana, IL, U.S.A aut NATIONALLICENCE 700 1- Korban Schuyler Department of Natural Resources and Environmental Sciences, University of Illinois, 310 Madigan Building, 1201 W. Gregory Drive, 61801, Urbana, IL, U.S.A aut NATIONALLICENCE 700 1- Krüger Jutta Federal Centre for Breeding Research on Cultivated Plants, Institute for Ornamental Plant Breeding, Bornkampsweg 31, 22916, Ahrensburg, Germany aut NATIONALLICENCE 700 1- Schmidt Hanna Federal Centre for Breeding Research on Cultivated Plants, Institute for Ornamental Plant Breeding, Bornkampsweg 31, 22916, Ahrensburg, Germany aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 94/2(1997-03-01), 175-182 0014-2336 94:2<175 1997 94 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer