caa a22 4500 477133487 CHVBK 20180405111710.0 cr unu---uuuuu 170330e19970301xx s 000 0 eng 10.1023/A:1002976408213 doi (NATIONALLICENCE)springer-10.1023/A:1002976408213 Characterization of a new prominent satellite DNA of Cucumis metuliferus and differential distribution of satellite DNA in cultivated and wild species of Cucumis and in related genera of Cucurbitaceae [Elektronische Daten] [Mariana Helm, Vera Hemleben] A new species-specific, tandemly arranged satellite (pMetSat) DNA was characterized by Southern hybridization, cloning and sequencing for Cucumis metuliferus, a cultivated species that originated in Africa. The 346 bp repeats share short stretches of remarkable sequence similarity with satellite DNA of other cultivated Cucumis species, in particular types I-IV of C. sativus (cucumber) and the 352 bp satellite of C. melo (melon), although no cross-hybridization occurs applying stringent conditions. Minor arrays of the respective repeat types were also detected in other less cultivated or wild Cucumis species by PCR amplification or long exposure of Southern blots. For the Cucumis species, the pattern of satellite distribution appears to reflect the taxonomic classification. This suggests that in ancestral species a set of differential but related repeats was already present. During speciation and cultivation, specific members of this set underwent amplifications and modifications. Differential distribution of small arrays of the Cucumis satellites within the genomes of other Cucurbitaceae (tribus Benincaseae, Trichosantheae, Sicyeae) is shown. Kluwer Academic Publishers, 1997 Cucurbitaceae nationallicence domestication nationallicence molecular evolution nationallicence plant breeding nationallicence repetitive DNA nationallicence Helm Mariana Department of Genetics, Biological Institute, University of Tübingen, Auf der Morgenstelle 28, 72076, Tübingen, Germany aut Hemleben Vera Department of Genetics, Biological Institute, University of Tübingen, Auf der Morgenstelle 28, 72076, Tübingen, Germany aut Euphytica Kluwer Academic Publishers 94/2(1997-03-01), 219-226 0014-2336 94:2<219 1997 94 10681 https://doi.org/10.1023/A:1002976408213 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002976408213 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Helm Mariana Department of Genetics, Biological Institute, University of Tübingen, Auf der Morgenstelle 28, 72076, Tübingen, Germany aut NATIONALLICENCE 700 1- Hemleben Vera Department of Genetics, Biological Institute, University of Tübingen, Auf der Morgenstelle 28, 72076, Tübingen, Germany aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 94/2(1997-03-01), 219-226 0014-2336 94:2<219 1997 94 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer