caa a22 4500 477133584 CHVBK 20180405111711.0 cr unu---uuuuu 170330e19971201xx s 000 0 eng 10.1023/A:1003152813333 doi (NATIONALLICENCE)springer-10.1023/A:1003152813333 Anthocyanin-flavone copigmentation in bluish purple flowers of Japanese garden iris (Iris ensata Thunb.) [Elektronische Daten] [T. Yabuya, M. Nakamura, T. Iwashina, M. Yamaguchi, T. Takehara] The in vitro identification of copigmentation was carried out using anthocyanins, such as malvidin 3RGac5G, petunidin 3RGac5G and delphinidin 3RGac5G and the flavone isovitexin. These are major pigments of Iris ensata. These anthocyanins brought about copigmentation, i.e., the bathochromic shift (bluing effect) of visible λ max due to increased concentrations of isovitexin, and 32 to 35 nm were estimated as the magnitude (Δλ max) of each shift. In addition, the absorption spectrum of 0.1 mM malvidin 3RGac5G, 0.07 mM petunidin 3RGac5G and 0.7 mM isovitexin mixture closely matched those of the fresh outer perianths of the bluish purple cultivars, ‘Suiten-isshoku', ‘Hekikai' and ‘Yakonotama' which belong to the malvidin 3RGac5G - petunidin 3RGac5G type of I. ensata. Therefore, these results indicated that the bluing effect on the flower color of the bluish purple cultivars of this species was caused at least in part by the copigmentation between these anthocyanins and the flavone isovitexin. For the copigment effects of isovitexin among malvidin 3RGac5G, petunidin 3RGac5G and delphinidin 3RGac5G, λ max and Δλ max of delphinidin 3RGac5G was slightly higher than those of malvidin 3RGac5G or petunidin 3RGac5G, and the copigmentation of delphinidin 3RGac5G was characterized by a higher concentration of isovitexin. Finally, the breeding for blue flowers due to copigmentation of delphinidin 3RGac5G with isovitexin in I. ensata was discussed. Kluwer Academic Publishers, 1997 Iris ensata nationallicence copigmentation nationallicence bluing effect nationallicence flower color breeding nationallicence Yabuya T. Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University, 889-21, Miyazaki, Japan aut Nakamura M. Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University, 889-21, Miyazaki, Japan aut Iwashina T. Tsukuba Botanical Garden, National Science Museum, 305, Tsukuba, Japan aut Yamaguchi M. Department of Food Science and Technology, Minami-Kyushu University, 884, Takanabe, Miyazaki, Japan aut Takehara T. Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University, 889-21, Miyazaki, Japan aut Euphytica Kluwer Academic Publishers 98/3(1997-12-01), 163-167 0014-2336 98:3<163 1997 98 10681 https://doi.org/10.1023/A:1003152813333 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003152813333 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yabuya T. Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University, 889-21, Miyazaki, Japan aut NATIONALLICENCE 700 1- Nakamura M. Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University, 889-21, Miyazaki, Japan aut NATIONALLICENCE 700 1- Iwashina T. Tsukuba Botanical Garden, National Science Museum, 305, Tsukuba, Japan aut NATIONALLICENCE 700 1- Yamaguchi M. Department of Food Science and Technology, Minami-Kyushu University, 884, Takanabe, Miyazaki, Japan aut NATIONALLICENCE 700 1- Takehara T. Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University, 889-21, Miyazaki, Japan aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 98/3(1997-12-01), 163-167 0014-2336 98:3<163 1997 98 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer