caa a22 4500 477133819 CHVBK 20180405111711.0 cr unu---uuuuu 170330e19971101xx s 000 0 eng 10.1023/A:1003104914242 doi (NATIONALLICENCE)springer-10.1023/A:1003104914242 Variability of gene expression in transgenic tobacco [Elektronische Daten] [Marie Mannerlöf, Paul Tenning] Variability in the expression of the introduced nptII gene was evaluated in transgenic tobacco. Expression analysis was performed on progeny plants of selfed primary transformants. Three different gene constructs were used, containing the nptII gene expressed by either 35S, heat shock protein (hsp80) or the hsp80 promoter including the TMV (Tobacco Mosaic Virus) omega translational enhancer element. Expression of the nptII gene in leaves collected from different developmental phases, varied up to twelve times. The variation in expression of NPTII between independent transformants, all transformed with the same gene construct was found to vary up to nine times. Expression of the nptII gene in the selfed progeny originating from one transformant varied up to four times. The 35S promoter showed a 50-100 fold higher expression of the nptII gene compared to the hsp80 promoter. The omega element enhanced the expression up to two times when compared to the same promoter without the omega element. Transformants containing multiple T-DNA inserts had generally a lower nptII expression compared to plants containing single T-DNA inserts. Implications of such variation in commercialized transgenic crops are discussed. Kluwer Academic Publishers, 1997 Agrobacterium tumefaciens nationallicence gene expression nationallicence neomycin phosphotransferase nationallicence Nicotiana tabacum nationallicence omega element nationallicence Mannerlöf Marie Hilleshög AB, P.O. BOX 302, S-261 23, Landskrona, Sweden aut Tenning Paul Hilleshög AB, P.O. BOX 302, S-261 23, Landskrona, Sweden aut Euphytica Kluwer Academic Publishers 98/1-2(1997-11-01), 133-139 0014-2336 98:1-2<133 1997 98 10681 https://doi.org/10.1023/A:1003104914242 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003104914242 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mannerlöf Marie Hilleshög AB, P.O. BOX 302, S-261 23, Landskrona, Sweden aut NATIONALLICENCE 700 1- Tenning Paul Hilleshög AB, P.O. BOX 302, S-261 23, Landskrona, Sweden aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 98/1-2(1997-11-01), 133-139 0014-2336 98:1-2<133 1997 98 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer