caa a22 4500 477133924 CHVBK 20180405111712.0 cr unu---uuuuu 170330e19970101xx s 000 0 eng 10.1023/A:1002945922280 doi (NATIONALLICENCE)springer-10.1023/A:1002945922280 The influence of cytokinins and ionic strength of Anderson's medium on shoot establishment and proliferation of evergreen azalea [Elektronische Daten] [Chi-Ni Hsia, Schuyler Korban] Shoot-tip explants of evergreen azalea cv. Fuchsia grown on Anderson's medium and containing different cytokinins produced the highest proliferation rate on a medium containing thidiazuron (TDZ). TDZ concentrations ranging from 0.23 to 2.3 μM resulted in both good bud-break rate (4 to 5) and shoot quality (> 0.5 cm in length). Adding 2.3 μM zeatin to Anderson's medium containing 0.23 or 2.3 μM TDZ increased the number of axillary shoots/explant. However, increasing the zeatin concentration to 4.6 μM resulted in a reduced shoot proliferation rate. A medium containing 1.15 μM TDZ and 2.3 μM zeatin resulted in an 18-fold increase for 'Fuchsia' and a 9-fold increase for 'Hino Crimson' after 6 weeks of culture. It was found that explants grown on a half-strength Anderson's medium with 87.6 mM sucrose generally had better shoot proliferation rate and shoot quality than at higher ionic strength. Kluwer Academic Publishers, 1997 Rhododendron nationallicence evergreen azalea nationallicence micropropagation nationallicence thidiazuron nationallicence Hsia Chi-Ni Perennial Plant Biogenetics Laboratory, Department of Natural Resources & Environmental Sciences, 310 Madigan Bldg., University of Illinois, 1201 W. Gregory, 61801, Urbana, IL, U.S.A aut Korban Schuyler Perennial Plant Biogenetics Laboratory, Department of Natural Resources & Environmental Sciences, 310 Madigan Bldg., University of Illinois, 1201 W. Gregory, 61801, Urbana, IL, U.S.A aut Euphytica Kluwer Academic Publishers 93/1(1997-01-01), 11-17 0014-2336 93:1<11 1997 93 10681 https://doi.org/10.1023/A:1002945922280 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002945922280 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hsia Chi-Ni Perennial Plant Biogenetics Laboratory, Department of Natural Resources & Environmental Sciences, 310 Madigan Bldg., University of Illinois, 1201 W. Gregory, 61801, Urbana, IL, U.S.A aut NATIONALLICENCE 700 1- Korban Schuyler Perennial Plant Biogenetics Laboratory, Department of Natural Resources & Environmental Sciences, 310 Madigan Bldg., University of Illinois, 1201 W. Gregory, 61801, Urbana, IL, U.S.A aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 93/1(1997-01-01), 11-17 0014-2336 93:1<11 1997 93 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer