caa a22 4500 477133932 CHVBK 20180405111712.0 cr unu---uuuuu 170330e19970101xx s 000 0 eng 10.1023/A:1002991721159 doi (NATIONALLICENCE)springer-10.1023/A:1002991721159 Analysis of reproductive isolation between pearl millet (Pennisetum glaucum (L.) R.Br.) and P. ramosum, P. schweinfurthii, P. squamulatum, Cenchrus ciliaris [Elektronische Daten] [L. Marchais, S. Tostain] Crosses between pearl millet lines and Pennisetum ramosum, P. schweinfurthii, P. squamulatum or Cenchrus ciliaris were observed for the frequency and development of zygotes, the possibility of embryo rescue, and the fertility of F1 hybrids obtained. Eight per cent of the ovules from diploid millet × P. ramosum crosses showed small embryos which could not be rescued. However, 59% of the ovules from tetraploid millet × P. ramosum crosses showed well-developed embryos that were easy to rescue 14 days after pollination. F1 hybrids were male sterile but female fertile when pollinated by diploid millet. Both diploid and tetraploid millet ovules showed the presence of hybrid zygotes after pollination with P. schweinfurthii at rates ranging from 25% to 45%. The diploid millet× P. schweinfurthii hybrid zygotes often developed almost normal seeds giving, without embryo rescue, totally sterile plants. The tetraploid millet × P. schweinfurthii hybrid embryos were normal but the endosperm was severely defective. A hybrid obtained by embryo rescue was totally sterile. A diploid millet-P. schweinfurthii amphidiploid was obtained by somatic embryogenesis associated with colchicine treatment during callogenesis. This amphiploid plant was male sterile, but gave many seeds when pollinated by a tetraploid millet and few seeds when pollinated by a diploid millet. P. squamulatum pollinating diploid millets produced proembryos with large undifferentiated endosperms in 73% of the ovules. A normal seed set was observed on tetraploid millets pollinated by P. squamulatum and the resulting F1 hybrids were partially male and female fertile. Backcrosses of these hybrids were much more fertile when pollination was from a tetraploid millet rather than from a diploid millet. C. ciliaris pollinating a diploid millet showed, in 60% of the ovules, proembryos and endosperms similar to those observed with P. squamulatum and no hybrid could be rescued. Crosses with a tetraploid millet could not be attempted due to the pistil-pollen incompatibility of tetraploid millets available with C. ciliaris. Ploidy levels of mating partners do not seem to influence pistil-pollen compatibility, but play a major role in post-zygotic abortion. With adequate ploidy levels of parents, and embryo rescue, it seems that the pearl millet gene pool can be considerably enlarged by germplasm from many other species. Kluwer Academic Publishers, 1997 interspecific hybridization nationallicence post-zygotic abortion nationallicence embryo rescue nationallicence amphiploidy nationallicence somatic embryogenesis nationallicence RAPD nationallicence Pennisetum nationallicence Marchais L. Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales, Orstom, 911 av. Agropolis, 34032, Montpellier, France aut Tostain S. Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales, Orstom, 911 av. Agropolis, 34032, Montpellier, France aut Euphytica Kluwer Academic Publishers 93/1(1997-01-01), 97-105 0014-2336 93:1<97 1997 93 10681 https://doi.org/10.1023/A:1002991721159 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002991721159 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marchais L. Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales, Orstom, 911 av. Agropolis, 34032, Montpellier, France aut NATIONALLICENCE 700 1- Tostain S. Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales, Orstom, 911 av. Agropolis, 34032, Montpellier, France aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 93/1(1997-01-01), 97-105 0014-2336 93:1<97 1997 93 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer