caa a22 4500 477133959 CHVBK 20180405111712.0 cr unu---uuuuu 170330e19970101xx s 000 0 eng 10.1023/A:1002946622376 doi (NATIONALLICENCE)springer-10.1023/A:1002946622376 Heritable improvement of frost tolerance in winter wheat by in vitro-selection of hydroxyproline-resistant proline overproducing mutants [Elektronische Daten] [K. Dörffling, H. Dörffling, G. Lesselich, E. Luck, C. Zimmermann, G. Melz, H.U. Jürgens] In a previous study (Dörffling et al., J. Plant Physiol. 142, 222-225, 1993) in vitro-selection and regeneration of hydroxyproline (Hyp)-resistant lines of winter wheat (Triticum aestivum cv. Jo 3063) with increased frost tolerance and increased proline contents was reported. In this study the heritability of these traits was investigated. The F 1progenies derived from the pollination of regenerated plants with pollen from wild type plants developed higher mean frost tolerance (lower LT 50values) and higher proline levels compared with in vitro-controls and wild type plants. In the F 2generation, which was obtained by self-pollination, segregation of the Hyp-lines in a 3:1 phenotypic ratio with regard to the traits frost tolerance and proline content was observed. Subsequent selection of extremes in the F 2yielded homozygous plants in the F 3generation. Lower LT 50values and increased proline contents in those F 3plants were significantly correlated. Furthermore, improved frost tolerance of one of the selected mutants was also observed in studies with whole plants in the F 4generation. These results, which are similar to results of parallel studies on winter barley, provide strong evidence for the heritability of the traits 'increased frost tolerance' and 'increased proline content' obtained by this in vitro-selection procedure. The mutation seems to be due to a single incompletely dominant gene. A comparison of frost tolerance values from F 3Hyp-progenies with those of seven standard varieties differing in frost tolerance indicates a considerable improvement of frost tolerance by the described in vitro-selection method. Kluwer Academic Publishers, 1997 frost tolerance nationallicence hydroxyproline (Hyp)-resistance nationallicence in vitro-selection nationallicence proline nationallicence Triticum aestivum nationallicence Dörffling K. Institute of General Botany, University of Hamburg, D-22609, Hamburg, Germany aut Dörffling H. Institute of General Botany, University of Hamburg, D-22609, Hamburg, Germany aut Lesselich G. Institute of General Botany, University of Hamburg, D-22609, Hamburg, Germany aut Luck E. Institute of General Botany, University of Hamburg, D-22609, Hamburg, Germany aut Zimmermann C. Institute of General Botany, University of Hamburg, D-22609, Hamburg, Germany aut Melz G. Ringstraße 10, D-18276, Gülzow, Germany aut Jürgens H.U. Federal Centre for Breeding Research on Cultivated Plants, D-18190, Groß Lüsewitz, Germany aut Euphytica Kluwer Academic Publishers 93/1(1997-01-01), 1-10 0014-2336 93:1<1 1997 93 10681 https://doi.org/10.1023/A:1002946622376 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002946622376 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dörffling K. Institute of General Botany, University of Hamburg, D-22609, Hamburg, Germany aut NATIONALLICENCE 700 1- Dörffling H. Institute of General Botany, University of Hamburg, D-22609, Hamburg, Germany aut NATIONALLICENCE 700 1- Lesselich G. Institute of General Botany, University of Hamburg, D-22609, Hamburg, Germany aut NATIONALLICENCE 700 1- Luck E. Institute of General Botany, University of Hamburg, D-22609, Hamburg, Germany aut NATIONALLICENCE 700 1- Zimmermann C. Institute of General Botany, University of Hamburg, D-22609, Hamburg, Germany aut NATIONALLICENCE 700 1- Melz G. Ringstraße 10, D-18276, Gülzow, Germany aut NATIONALLICENCE 700 1- Jürgens H.U. Federal Centre for Breeding Research on Cultivated Plants, D-18190, Groß Lüsewitz, Germany aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 93/1(1997-01-01), 1-10 0014-2336 93:1<1 1997 93 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer