caa a22 4500 477133975 CHVBK 20180405111712.0 cr unu---uuuuu 170330e19970101xx s 000 0 eng 10.1023/A:1002995822067 doi (NATIONALLICENCE)springer-10.1023/A:1002995822067 Sujatha M. Genetics and Cytogenetics Section, Directorate of Oilseeds Research, Rajendranagar, 500 030, Hyderabad, India aut In vitro adventitious shoot regeneration for effective maintenance of male sterile niger Guizotia abyssinica (L.f.) Cass.) [Elektronische Daten] [M. Sujatha] A rapid and efficient method of in vitro plant regeneration for large scale propagation of male sterile plants of niger (Guizotia abyssinica) was developed. Leaf segments from mature plants were cultured on Murashige & Skoog's basal medium (MS) supplemented with N6-benzyladenine (BA) and kinetin individually and in combination with low concentrations of indole-3-butyric acid (IBA); and α-Naphthaleneacetic acid (NAA). Prolific direct adventitious shoot regeneration occurred on most of the media tested. The best response in terms of frequency of shoot regeneration and the number of shoots per leaf explant was observed on medium supplemented with 2.22 μM BA. Transfer of shoot bud clusters to fresh medium with same composition promoted further multiplication of the shoot buds, while medium with reduced BA concentration (0.89 μM) facilitated shoot elongation. Shoots that were rooted on half-strength MS medium gelled with 0.2 or 0.4% agar and supplemented with 4.9μ M IBA survived with a frequency of 61.36% on transfer to ex vitrum conditions. Field evaluation of the regenerants revealed the genetic stability of the plantlets and are being used in breeding of experimental hybrids. Kluwer Academic Publishers, 1997 Adventitious regeneration nationallicence Guizotia abyssinica nationallicence male sterility nationallicence niger nationallicence tissue culture nationallicence Euphytica Kluwer Academic Publishers 93/1(1997-01-01), 89-95 0014-2336 93:1<89 1997 93 10681 https://doi.org/10.1023/A:1002995822067 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002995822067 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Sujatha M. Genetics and Cytogenetics Section, Directorate of Oilseeds Research, Rajendranagar, 500 030, Hyderabad, India aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 93/1(1997-01-01), 89-95 0014-2336 93:1<89 1997 93 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer