caa a22 4500 477134017 CHVBK 20180405111712.0 cr unu---uuuuu 170330e19970201xx s 000 0 eng 10.1023/A:1002957800957 doi (NATIONALLICENCE)springer-10.1023/A:1002957800957 Chromosome doubling of Allium fistulosum × A. cepa interspecific F1 hybrids through colchicine treatment of regenerating callus [Elektronische Daten] [Ping Song, Wanhee Kang, Ellen Peffley] Regenerating calluses of Allium fistulosum × A. cepa interspecific F1 hybrids were treated in vitro with colchicine. A factorial experiment was designed to test the effects of colchicine concentration and time on the recovery of tetraploid plants from in vitro-colchicine-treated calluses. Shoot production of regenerating calluses following in vitro colchicine treatment decreased with increasing colchicine concentration and treatment time. Cytological analyses of root tip cells from regenerated plantlets showed that chromosomes of control plantlets (not treated with colchicine) were not doubled. Chromosome number of some plantlets regenerated from in vitro-colchicine-treated calluses were doubled, resulting in tetraploids. Calluses treated with 0.1 or 0.2% colchicine in BDS liquid medium for 48 or 72 hours yielded the highest numbers of tetraploid plantlets. Chromosome bridges at anaphase or early telophase were observed in diploid and tetraploid plants; their potential use is discussed. These results demonstrate that in vitro-colchicine treatment of regenerating calluses of interspecific F1 hybrids is effective in recovering tetraploid plants. Kluwer Academic Publishers, 1997 Allium fistulosum nationallicence Allium cepa nationallicence allotetraploids nationallicence amphidiploids nationallicence breeding nationallicence genetics nationallicence onion nationallicence tetraploids nationallicence Song Ping Department of Plant and Soil Science, Texas Technical University, 79409-2122, Lubbock, TX, U.S.A aut Kang Wanhee Department of Plant and Soil Science, Texas Technical University, 79409-2122, Lubbock, TX, U.S.A aut Peffley Ellen Department of Plant and Soil Science, Texas Technical University, 79409-2122, Lubbock, TX, U.S.A aut Euphytica Kluwer Academic Publishers 93/3(1997-02-01), 257-262 0014-2336 93:3<257 1997 93 10681 https://doi.org/10.1023/A:1002957800957 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002957800957 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Ping Department of Plant and Soil Science, Texas Technical University, 79409-2122, Lubbock, TX, U.S.A aut NATIONALLICENCE 700 1- Kang Wanhee Department of Plant and Soil Science, Texas Technical University, 79409-2122, Lubbock, TX, U.S.A aut NATIONALLICENCE 700 1- Peffley Ellen Department of Plant and Soil Science, Texas Technical University, 79409-2122, Lubbock, TX, U.S.A aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 93/3(1997-02-01), 257-262 0014-2336 93:3<257 1997 93 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer