caa a22 4500 477134076 CHVBK 20180405111712.0 cr unu---uuuuu 170330e19970201xx s 000 0 eng 10.1023/A:1002958623171 doi (NATIONALLICENCE)springer-10.1023/A:1002958623171 RFLP analysis of cytoplasmic male-sterile lines of pigeonpea [Cajanus cajan (L.) Millsp.] developed by interspecific crosses [Elektronische Daten] [S. Sivaramakrishnan, K. Seetha, A. Nageshwar Rao, Laxman Singh] Total DNA from three putative cytoplasmic male sterile (CMS) progenies derived from crosses between the wild species Cajanus sericeus and the cultivated species Cajanus cajan, five C. cajan, one accession of C. sericeus and two genetic male sterile lines of pigeonpea were compared for their RFLP patterns using maize mitochondrial DNA (mtDNA) specific probes. Three putative cytoplasmic male sterile (CMS) progenies from the multiple cross genome transfer of pigeonpea lines (CMS 7-1, CMS 12-3, and CMS 33-1) showed hybridization patterns identical to that of C. sericeus when DNA was digested with EcoRI and HindIII and probed with maize mtDNA clones. The results suggested that these putative CMS progenies have the mitochondria of the female wild species parent. The hybridization patterns of the three male parental lines used in the development of the CMS progenies were similar in all the restriction enzyme-probe combinations except HindIII-atp6. The genetic male sterile lines, MS Prabhat and QMS 1 differed from each other in their hybridization pattern. The genomic DNA hybridization pattern of HindIII digested DNA from ICPL 87 differed from the other pigeonpea lines when probed with the maize mtDNA clones. The cluster analysis of the hybridization data suggested the occurrence of variation in the mitochondrial genome even among the cultivated species. Kluwer Academic Publishers, 1997 Cajanus sericeus nationallicence CMS nationallicence mtDNA nationallicence RFLP nationallicence pigeonpea nationallicence Cajanus cajan nationallicence Sivaramakrishnan S. Cellular and Molecular Biology Division, India aut Seetha K. Cellular and Molecular Biology Division, India aut Nageshwar Rao A. Genetic Enhancement Division, International Crops Institute for the Semi-Arid Tropics (ICRISAT), 502 324, Andhra Pradesh, India aut Singh Laxman Genetic Enhancement Division, International Crops Institute for the Semi-Arid Tropics (ICRISAT), 502 324, Andhra Pradesh, India aut Euphytica Kluwer Academic Publishers 93/3(1997-02-01), 307-312 0014-2336 93:3<307 1997 93 10681 https://doi.org/10.1023/A:1002958623171 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002958623171 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sivaramakrishnan S. Cellular and Molecular Biology Division, India aut NATIONALLICENCE 700 1- Seetha K. Cellular and Molecular Biology Division, India aut NATIONALLICENCE 700 1- Nageshwar Rao A. Genetic Enhancement Division, International Crops Institute for the Semi-Arid Tropics (ICRISAT), 502 324, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Singh Laxman Genetic Enhancement Division, International Crops Institute for the Semi-Arid Tropics (ICRISAT), 502 324, Andhra Pradesh, India aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 93/3(1997-02-01), 307-312 0014-2336 93:3<307 1997 93 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer