caa a22 4500 477134106 CHVBK 20180405111712.0 cr unu---uuuuu 170330e19970201xx s 000 0 eng 10.1023/A:1002965322262 doi (NATIONALLICENCE)springer-10.1023/A:1002965322262 Wide hybridization between wheat (Triticum L.) and lymegrass (Leymus Hochst.) [Elektronische Daten] [Kesara Anamthawat-Jónsson, Sigrídur Bödvarsdóttir, Birkir Bragason, Jón Gudmundsson, P.K. Martin, R.M.D. Koebner] A total of 240 F1 hybrids was made beween wheat (Triticum aestivum L. em. Thell. (2n = 6x = 42) and T. carthlicum Nevski (2n = 4x = 28)) and perennial lymegrass (North European Leymus arenarius (L.) Hochst. (2n = 8x = 56) and North American L. mollis (Trin.) Pilger (2n = 4x = 28)). The wide crosses yielded embryos in 20% of caryopses and 96% of the embryos developed into normal hybrid plants. The hybrids were vegetatively vigorous, with evidence of the Leymus rhizomatous habit. Those deriving from L. arenarius survived overwintering in Iceland, but the hybrids L. mollis did not, whereas in a milder environment, both showed perenniality. Cytogenetic analysis of root tip cells before the plants were treated with colchicine showed that 21 out of 28 hybrids investigated had chromosome mosaics, with a population of both amphihaploid and amphidiploid cells. This spontaneous doubling of somatic chromosomes occurred in all cross combinations, with the highest average frequency of diploid cells (28%) in T. carthlicum × L. arenarius crosses. A few selfed seeds have been obtained from a T. aestivum × L. arenarius hybrid. All the hybrids were treated twice with colchicine, but the treatment appeared to have little or no effect on the frequency of chromosome doubling in the hybrids deriving from T. aestivum. The frequency of diploid cells, however, increased significantly (e.g. to 80%) in the hybrids deriving from the T. carthlicum parent. Genomic in situ hybridization confirmed the hybridity of the plants and showed that the hybrids were amphiploids containing genomes of both wheat and lymegrass. In situ hybridization using ribosomal DNA probe differentiated chromosomes of L. mollis, L. arenarius from those of wheat. The hybrids are being backcrossed with lymegrass pollen, aiming to domesticate the wild, perennial species. Kluwer Academic Publishers, 1997 lymegrass nationallicence Triticum nationallicence wheat nationallicence wide hybrids nationallicence cytogenetics nationallicence in situ hybridization nationallicence Anamthawat-Jónsson Kesara Agricultural Research Institute, IS-112, Keldnaholt, Reykjavík, Iceland aut Bödvarsdóttir Sigrídur Agricultural Research Institute, IS-112, Keldnaholt, Reykjavík, Iceland aut Bragason Birkir Agricultural Research Institute, IS-112, Keldnaholt, Reykjavík, Iceland aut Gudmundsson Jón Agricultural Research Institute, IS-112, Keldnaholt, Reykjavík, Iceland aut Martin P.K. Cereals Research Department, Cambridge Laboratory, John Innes Centre, NR4 7UJ, Norwich, United Kingdom aut Koebner R.M.D. Cereals Research Department, Cambridge Laboratory, John Innes Centre, NR4 7UJ, Norwich, United Kingdom aut Euphytica Kluwer Academic Publishers 93/3(1997-02-01), 293-300 0014-2336 93:3<293 1997 93 10681 https://doi.org/10.1023/A:1002965322262 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002965322262 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Anamthawat-Jónsson Kesara Agricultural Research Institute, IS-112, Keldnaholt, Reykjavík, Iceland aut NATIONALLICENCE 700 1- Bödvarsdóttir Sigrídur Agricultural Research Institute, IS-112, Keldnaholt, Reykjavík, Iceland aut NATIONALLICENCE 700 1- Bragason Birkir Agricultural Research Institute, IS-112, Keldnaholt, Reykjavík, Iceland aut NATIONALLICENCE 700 1- Gudmundsson Jón Agricultural Research Institute, IS-112, Keldnaholt, Reykjavík, Iceland aut NATIONALLICENCE 700 1- Martin P.K. Cereals Research Department, Cambridge Laboratory, John Innes Centre, NR4 7UJ, Norwich, United Kingdom aut NATIONALLICENCE 700 1- Koebner R.M.D. Cereals Research Department, Cambridge Laboratory, John Innes Centre, NR4 7UJ, Norwich, United Kingdom aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 93/3(1997-02-01), 293-300 0014-2336 93:3<293 1997 93 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer