caa a22 4500 477134246 CHVBK 20180405111713.0 cr unu---uuuuu 170330e19970101xx s 000 0 eng 10.1023/A:1002996818918 doi (NATIONALLICENCE)springer-10.1023/A:1002996818918 Nitrate reductase and nitrite reductase as targets to study gene silencing phenomena in transgenic plants [Elektronische Daten] [Hervé Vaucheret, Jean-Christophe Palauqui, Philippe Mourrain, Taline Elmayan] Nitrate assimilation is a fundamental function in any plant including those that can fix atmospheric nitrogen in symbiosis with soil bacteria. In recent years, attempts have been made to understand the biological significance of the complex regulation of this pathway using genetic engineering techniques. Transgenic plants that either over- or under-express genes of the nitrate assimilation pathway were created in order to determine whether such directed changes affect the regulation of the metabolism. Apart from interesting physiological results, unexpected gene silencing phenomena have been observed resulting from the introduction of five different transgenes derived from either the tobacco Nia or Nii genes encoding nitrate reductase and nitrite reductase, respectively. In this review, each of these five silencing phenomena is described, with the emphasis on the advantages provided by the use of both Nia and Nii genes to analyze the molecular and genetic basis of gene silencing in transgenic plants. Kluwer Academic Publishers, 1997 nitrate reductase nationallicence nitrite reductase nationallicence transgene nationallicence gene silencing nationallicence tobacco nationallicence Vaucheret Hervé Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut Palauqui Jean-Christophe Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut Mourrain Philippe Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut Elmayan Taline Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut Euphytica Kluwer Academic Publishers 93/2(1997-01-01), 195-200 0014-2336 93:2<195 1997 93 10681 https://doi.org/10.1023/A:1002996818918 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002996818918 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vaucheret Hervé Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Palauqui Jean-Christophe Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Mourrain Philippe Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Elmayan Taline Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 93/2(1997-01-01), 195-200 0014-2336 93:2<195 1997 93 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer