caa a22 4500 477134408 CHVBK 20180405111713.0 cr unu---uuuuu 170330e19970901xx s 000 0 eng 10.1023/A:1003041006157 doi (NATIONALLICENCE)springer-10.1023/A:1003041006157 Selection of vigorous and fertile S-homo- and heterozygous tester clones from self-incompatible diploid potato, Solanum tuberosum L [Elektronische Daten] [R. Eijlander, M.S. Ramanna, E. Jacobsen] For the selection of diploid (2n = 2x = 24) potato (Solanum tuberosum) genotypes that are useful for the molecular and genetic analysis of the phenomenon of gametophytic self-incompatibility, three different types of basic populations were investigated. These populations were derived from three primary dihaploid clones, G609, G254 and B16, which possessed the S-allele combinations S1S2, S1S3 and S3S4 respectively. In order to select highly vigorous, profusely flowering, fertile and tuberising progenies, three types of populations, derived from the above mentioned diploid genotypes, were screened for performance and classified for the expression of self-incompatibility. Although the selection for well defined S-genotypes was sometimes complicated due to the occurrence of pseudo-compatibility and of a self-compatibilising factor, the use of a combination of criteria, viz., Iso Electric Focusing (IEF), pollen tube growth in the styles and the extent of berry and seed set made the selection of sufficient representatives of all six types of S-heterozygotes (S1S2, S1S3, S1S4, S2S3, S2S4 and S3S4) possible. After evaluating the strength of the self-incompatibility reaction in these heterozygotes, those with high expression were selfed, and intercrossed within their S-allele incompatibility group through the method of counterfeit pollination. In these progenies, well-performing S-homozygotes (S1S1; S2S2; S3S3; S4S4) for all four S-alleles with high expression of self-incompatibility were selected. As a result, all possible S-homo- and heterozygous genotypes with a predictable type of self-incompatibility are available and maintained both vegetatively and as botanical seed. The development of this material has paved the way for more critical analysis of molecular factors involved in self-incompatibility in diploid potato. Kluwer Academic Publishers, 1997 Solanum tuberosum nationallicence potato nationallicence gametophytic self-incompatibility nationallicence S-alleles nationallicence pseudo-compatibility nationallicence counterfeit pollination nationallicence homozygotes nationallicence embryo spot nationallicence Eijlander R. Eps, the Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut Ramanna M.S. Eps, the Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut Jacobsen E. Eps, the Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut Euphytica Kluwer Academic Publishers 97/1(1997-09-01), 97-111 0014-2336 97:1<97 1997 97 10681 https://doi.org/10.1023/A:1003041006157 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003041006157 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Eijlander R. Eps, the Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Ramanna M.S. Eps, the Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Jacobsen E. Eps, the Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 97/1(1997-09-01), 97-111 0014-2336 97:1<97 1997 97 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer