caa a22 4500 477134432 CHVBK 20180405111713.0 cr unu---uuuuu 170330e19971101xx s 000 0 eng 10.1023/A:1002966824988 doi (NATIONALLICENCE)springer-10.1023/A:1002966824988 Doubled haploid production of sunflower (Helianthus annuus L.) through irradiated pollen-induced parthenogenesis [Elektronische Daten] [M. Todorova, P. Ivanov, P. Shindrova, M. Christov, I. Ivanova] Induced parthenogenesis as a possible method of haploidization in sunflower (H. annuus L.) was tested in previous investigations (Todorova et al., 1994) and conditions for reproducible regeneration of gynogenic doubled haploids were established by the present work. Forty eight treatments were studied, involving four pollen donors and four recipient hybrids. Pollen was irradiated with doses of 300 Gy, 600 Gy and 900 Gy. In total, 2279 embryos were cultivated in vitro of which 1107 plants were obtained and 582 of them produced seeds after selfing. The ploidy level of the regenerants was evaluated at the two - three leaf stage and 296 of the plantlets obtained were haploids. Some of them underwent spontaneous diploidization, the others were treated with colchicine solution for chromosome doubling. The diploid plantlets were checked for their gynogenic origin by genetical and biochemical methods. The effectiveness of the method expressed as the number of agronomically useful DH lines to the number obtained that were fertile and resistant to downy mildew, branched and unbranched plants was 8,6% on average. Kluwer Academic Publishers, 1997 Helianthus annuus haploid induction nationallicence induced parthenogenesis nationallicence irradiated pollen nationallicence Todorova M. Iws ‘Dobroudja', 9520, General Toshevo, Bulgaria aut Ivanov P. Iws ‘Dobroudja', 9520, General Toshevo, Bulgaria aut Shindrova P. Iws ‘Dobroudja', 9520, General Toshevo, Bulgaria aut Christov M. Iws ‘Dobroudja', 9520, General Toshevo, Bulgaria aut Ivanova I. Iws ‘Dobroudja', 9520, General Toshevo, Bulgaria aut Euphytica Kluwer Academic Publishers 97/3(1997-11-01), 249-254 0014-2336 97:3<249 1997 97 10681 https://doi.org/10.1023/A:1002966824988 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002966824988 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Todorova M. Iws ‘Dobroudja', 9520, General Toshevo, Bulgaria aut NATIONALLICENCE 700 1- Ivanov P. Iws ‘Dobroudja', 9520, General Toshevo, Bulgaria aut NATIONALLICENCE 700 1- Shindrova P. Iws ‘Dobroudja', 9520, General Toshevo, Bulgaria aut NATIONALLICENCE 700 1- Christov M. Iws ‘Dobroudja', 9520, General Toshevo, Bulgaria aut NATIONALLICENCE 700 1- Ivanova I. Iws ‘Dobroudja', 9520, General Toshevo, Bulgaria aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 97/3(1997-11-01), 249-254 0014-2336 97:3<249 1997 97 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer