caa a22 4500 477134572 CHVBK 20180405111714.0 cr unu---uuuuu 170330e19971101xx s 000 0 eng 10.1023/A:1003045929279 doi (NATIONALLICENCE)springer-10.1023/A:1003045929279 Selection of Al-resistant plants from a sensitive rice cultivar, using somaclonal variation, in vitro and hydroponic cultures [Elektronische Daten] [Véronique Van Sint Jan, Cristiane Costa de Macedo, Jean-Marie Kinet, Jules Bouharmont] A selection procedure is described that isolated Al-resistant individuals from a sensitive rice (Oryza sativa L.) genotype. Somaclonal variation was used as the only source of variability and selection pressures were applied at both callus and regenerated plant levels. When 10 week-old, embryo-derived calli were submitted to different Al stresses for a period of 20 weeks. After this in vitro stress period, selected calli were grown during 18 weeks, without selection pressure, on regeneration media. Selection pressures were applied on regenerated plants (R0), and 9 R0 plants which produced seeds were selected. The transmission of the Al-resistant character to the R1, R2, R3 and R4 generations was then investigated, and three plant lines which had an increased percentage of Al-resistant plants till the fourth generation of self-pollination, were identified. One was from a callus maintained on an Al-free medium. The efficiency of in vitro selection pressures was therefore debated. Kluwer Academic Publishers, 1997 Oryza sativa nationallicence rice nationallicence aluminium-resistance nationallicence selection nationallicence stress nationallicence in vitro nationallicence hydroponic cultures nationallicence Van Sint Jan Véronique Laboratoire de Cytogénétique, Université Catholique de Louvain, Place Croix du Sud, 5 b13, B-1348, Louvain-la-Neuve, Belgium aut Costa de Macedo Cristiane Laboratoire de Cytogénétique, Université Catholique de Louvain, Place Croix du Sud, 5 b13, B-1348, Louvain-la-Neuve, Belgium aut Kinet Jean-Marie Laboratoire de Cytogénétique, Université Catholique de Louvain, Place Croix du Sud, 5 b13, B-1348, Louvain-la-Neuve, Belgium aut Bouharmont Jules Laboratoire de Cytogénétique, Université Catholique de Louvain, Place Croix du Sud, 5 b13, B-1348, Louvain-la-Neuve, Belgium aut Euphytica Kluwer Academic Publishers 97/3(1997-11-01), 303-310 0014-2336 97:3<303 1997 97 10681 https://doi.org/10.1023/A:1003045929279 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003045929279 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van Sint Jan Véronique Laboratoire de Cytogénétique, Université Catholique de Louvain, Place Croix du Sud, 5 b13, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Costa de Macedo Cristiane Laboratoire de Cytogénétique, Université Catholique de Louvain, Place Croix du Sud, 5 b13, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Kinet Jean-Marie Laboratoire de Cytogénétique, Université Catholique de Louvain, Place Croix du Sud, 5 b13, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Bouharmont Jules Laboratoire de Cytogénétique, Université Catholique de Louvain, Place Croix du Sud, 5 b13, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 97/3(1997-11-01), 303-310 0014-2336 97:3<303 1997 97 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer