caa a22 4500 477134645 CHVBK 20180405111714.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1003030913308 doi (NATIONALLICENCE)springer-10.1023/A:1003030913308 Correlation of ribonuclease zymograms and incompatibility genotypes in almond [Elektronische Daten] [R. Bošković, K.R. Tobutt, I. Batlle, H. Duval] Proteins were extracted from styles of 29 self-incompatible cultivars of almond and separated using non-equilibrium pH gradient electro-focusing, and the gels were stained for ribonuclease activity. Mutually incompatible cultivars had similar banding patterns and, for the 24 cultivars already genotyped in France or California, the bands correlated well with the reported alleles. The band corresponding to S1 of the French labelling system was indistinguishable from that corresponding to Sb of the Californian labelling system, and a controlled cross confirmed that these alleles are identical. The band corresponding to the Californian Sa was distinct from the bands corresponding to French alleles and, to harmonise the allele labels, it was redesignated S5. The genotypes of five uncharacterised self-incompatible cultivars were inferred from zymograms as follows: ‘Desmayo Largueta' and ‘Glorieta', S1S5, ‘Masbovera', S1S9, ‘Tarragones', S2S9, and ‘Tokyo', S6S7. The alleles designated S6 and S9 have not previously been reported. Nine self-compatible cultivars or selections were analysed, and each showed a band corresponding to an incompatibility allele as well as a common band; however, the correspondence of this common band to Sf, the allele for self-compatibility, is unproven. Kluwer Academic Publishers, 1997 Almond nationallicence incompatibility nationallicence non-equilibrium pH gradient electro-focusing nationallicence Prunus dulcis nationallicence ribonuclease nationallicence self-compatibility nationallicence Bošković R. Horticulture Research International, East Malling, West Malling, ME19 6BJ, Kent, UK aut Tobutt K.R. Horticulture Research International, East Malling, West Malling, ME19 6BJ, Kent, UK aut Batlle I. Centre de Mas Bové, Institut de Recerca i Tecnologia Agroalimentàries, Apartat 415, E-43280, Reus, Spain aut Duval H. Station de Recherches Fruitières Méditerranéennes, Institut National de la Recherche Agronomique, 84914, Avignon Cedex 9, France aut Euphytica Kluwer Academic Publishers 97/2(1997-10-01), 167-176 0014-2336 97:2<167 1997 97 10681 https://doi.org/10.1023/A:1003030913308 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003030913308 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bošković R. Horticulture Research International, East Malling, West Malling, ME19 6BJ, Kent, UK aut NATIONALLICENCE 700 1- Tobutt K.R. Horticulture Research International, East Malling, West Malling, ME19 6BJ, Kent, UK aut NATIONALLICENCE 700 1- Batlle I. Centre de Mas Bové, Institut de Recerca i Tecnologia Agroalimentàries, Apartat 415, E-43280, Reus, Spain aut NATIONALLICENCE 700 1- Duval H. Station de Recherches Fruitières Méditerranéennes, Institut National de la Recherche Agronomique, 84914, Avignon Cedex 9, France aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 97/2(1997-10-01), 167-176 0014-2336 97:2<167 1997 97 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer