caa a22 4500 477134661 CHVBK 20180405111714.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1003097913349 doi (NATIONALLICENCE)springer-10.1023/A:1003097913349 Identification of RAPD markers for resistance to coffee berry disease, Colletotrichum kahawae, in arabica coffee [Elektronische Daten] [Charles Agwanda, Philippe Lashermes, Pierre Trouslot, Marie-Christine Combes, André Charrier] Resistance to Coffee Berry Disease (CBD) in Arabica coffee is controlled by at least three genes which are present in the varieties Hibrido de Timor (T gene), Catimor (T gene), Rume Sudan (R and k genes) and K7 (k gene). Hibrido de Timor, Catimor and Rume Sudan are genetically distant from most of the commercial cultivars, and the utilisation of molecular markers would greatly improve the efficiency of breeding programmes concerned with CBD resistance. The objectives of the present work were therefore: (1) to identify random amplified polymorphic DNA (RAPD) markers associated with CBD resistance and (2) to identify markers which could be used to select against the genetic background of the resistance donors. Identification of RAPD markers was carried out in three steps. The first step involved the comparison of the RAPD profiles between the susceptible cultivars and the resistant donors. This was followed by comparison of the RAPD profiles between resistant and susceptible types of each donor variety. The final step involved assay of the resistance markers in the first and the second backcrosses between these donors and the recurrent parent. High genetic variability was demonstrated in Catimor, and to some extent in Rume Sudan. Three RAPD markers were shown to be closely associated to the T gene. Attempts to identify markers associated with the R and k genes were less rewarding. The implications of the current observations in relation to breeding for CBD resistance in Arabica coffee are discussed. Kluwer Academic Publishers, 1997 arabica coffee nationallicence coffee berry disease nationallicence marker nationallicence random amplified polymorphic DNA nationallicence resistance nationallicence Agwanda Charles Coffee Research Foundation, P. O. Box 4, Ruiru, Kenya aut Lashermes Philippe Orstom, Laboratoire de Resource génétiques et d'amélioration des plantes tropicales, BP 5045, F-34032, Montpellier, France aut Trouslot Pierre Orstom, Laboratoire de Resource génétiques et d'amélioration des plantes tropicales, BP 5045, F-34032, Montpellier, France aut Combes Marie-Christine Orstom, Laboratoire de Resource génétiques et d'amélioration des plantes tropicales, BP 5045, F-34032, Montpellier, France aut Charrier André Ensa.M, place Viala, F-34060, Montpellier, France aut Euphytica Kluwer Academic Publishers 97/2(1997-10-01), 241-248 0014-2336 97:2<241 1997 97 10681 https://doi.org/10.1023/A:1003097913349 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003097913349 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Agwanda Charles Coffee Research Foundation, P. O. Box 4, Ruiru, Kenya aut NATIONALLICENCE 700 1- Lashermes Philippe Orstom, Laboratoire de Resource génétiques et d'amélioration des plantes tropicales, BP 5045, F-34032, Montpellier, France aut NATIONALLICENCE 700 1- Trouslot Pierre Orstom, Laboratoire de Resource génétiques et d'amélioration des plantes tropicales, BP 5045, F-34032, Montpellier, France aut NATIONALLICENCE 700 1- Combes Marie-Christine Orstom, Laboratoire de Resource génétiques et d'amélioration des plantes tropicales, BP 5045, F-34032, Montpellier, France aut NATIONALLICENCE 700 1- Charrier André Ensa.M, place Viala, F-34060, Montpellier, France aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 97/2(1997-10-01), 241-248 0014-2336 97:2<241 1997 97 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer