caa a22 4500 47713470X CHVBK 20180405111714.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1002949813052 doi (NATIONALLICENCE)springer-10.1023/A:1002949813052 Identification of polymorphic DNA markers in cultivated peanut (Arachis hypogaea L.) [Elektronische Daten] [Guohao He, Channapatna Prakash] The detection of DNA polymorphism in cultivated peanut (Arachis hypogaea L.) is reported here for the first time. The DNA amplification fingerprinting (DAF) and amplified fragment length polymorphism (AFLP) approaches were tested for their potential to detect genetic variation in peanut. The AFLP approach was more efficient as 43% of the primer combinations detected polymorphic DNA markers in contrast to 3% with the DAF approach. However, the number of polymorphic bands identified using primers selected in both approaches was comparable. In the DAF study, when 559 primers of varying types were screened, 17 (mostly 10-mer types) detected polymorphism producing an average of 3.7 polymorphic bands per primer with a total of 63 polymorphic markers. In the AFLP study, when 64 primer combinations (three selective nucleotides) corresponding to restriction enzymes Eco RI and Mse I were screened, 28 detected polymorphism. On an average, 6.7% of bands obtained from these 28 primer pairs were polymorphic resulting in a total of 111 AFLP markers. Our results demonstrate that both AFLP and DAF approaches can be employed to generate DNA markers in peanut and thus have potential in the marker-assisted genetic improvement and germplasm evaluation of this economically important crop. Kluwer Academic Publishers, 1997 amplified fragment length polymorphism (AFLP) markers nationallicence DNA amplification fingerprinting (DAF) nationallicence groundnut nationallicence polymerase chain reaction (PCR) nationallicence random amplified polymorphic DNA (RAPD) markers nationallicence He Guohao Center for Plant Biotechnology Research, Tuskegee University, School of Agriculture and Home Economics, Milbank Hall, 36088, Tuskegee, AL, U.S.A aut Prakash Channapatna Center for Plant Biotechnology Research, Tuskegee University, School of Agriculture and Home Economics, Milbank Hall, 36088, Tuskegee, AL, U.S.A aut Euphytica Kluwer Academic Publishers 97/2(1997-10-01), 143-149 0014-2336 97:2<143 1997 97 10681 https://doi.org/10.1023/A:1002949813052 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002949813052 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He Guohao Center for Plant Biotechnology Research, Tuskegee University, School of Agriculture and Home Economics, Milbank Hall, 36088, Tuskegee, AL, U.S.A aut NATIONALLICENCE 700 1- Prakash Channapatna Center for Plant Biotechnology Research, Tuskegee University, School of Agriculture and Home Economics, Milbank Hall, 36088, Tuskegee, AL, U.S.A aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 97/2(1997-10-01), 143-149 0014-2336 97:2<143 1997 97 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer