caa a22 4500 477134718 CHVBK 20180405111714.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1002919131805 doi (NATIONALLICENCE)springer-10.1023/A:1002919131805 Development of the components of a cytoplasmic male sterility hybrid system in rye through anther culture [Elektronische Daten] [Earl Bicar, Norman Darvey] Anther culture was applied as a method to develop the essential components of a cytoplasmic male sterility hybrid system in rye (Secale cereale L.). These components are the male sterile seed parent (A line), its isogenic maintainer counterpart (B line) and the restorer pollen parent (R line). Australian rye cultivars were crossed reciprocally to the cultivar ‘Luchs' which carries the Pampa male sterile cytoplasm (cms-P). Anthers of the F1s in the cms-P cytoplasm (primary cross) and their reciprocals in the normal cytoplasm (reciprocal cross) were cultured in a modified C17 medium. Male sterile and male fertile doubled haploids were obtained from the anther culture of the F1s in the cms-P cytoplasm. Testcrosses indicated that the male sterile doubled haploids were A lines and the male fertile doubled haploids were R lines (restorers). The anther culture of genotypes in the normal cytoplasm (reciprocal cross) gave all male fertile doubled haploids. Testcrosses indicated that the male fertile doubled haploids were R lines (restorers) in the normal cytoplasm. The expected maintainer B lines were not identified because of the limited number of doubled haploids obtained from the anther culture of reciprocal crosses. Experimental single cross hybrids between male sterile and restorer male fertile doubled haploids showed high levels of heterosis. The results of this study have significant economic implications especially in the production of hybrids in several species. Kluwer Academic Publishers, 1997 secale cereale nationallicence anther culture nationallicence cereal rye nationallicence cytoplasmic male sterility nationallicence doubled haploids nationallicence hybrids nationallicence Bicar Earl University of Sydney Plant Breeding Institute, Cobbitty, 107 Cobbitty Road, 2570, Cobbitty, NSW, Australia aut Darvey Norman University of Sydney Plant Breeding Institute, Cobbitty, 107 Cobbitty Road, 2570, Cobbitty, NSW, Australia aut Euphytica Kluwer Academic Publishers 97/2(1997-10-01), 151-160 0014-2336 97:2<151 1997 97 10681 https://doi.org/10.1023/A:1002919131805 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002919131805 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bicar Earl University of Sydney Plant Breeding Institute, Cobbitty, 107 Cobbitty Road, 2570, Cobbitty, NSW, Australia aut NATIONALLICENCE 700 1- Darvey Norman University of Sydney Plant Breeding Institute, Cobbitty, 107 Cobbitty Road, 2570, Cobbitty, NSW, Australia aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 97/2(1997-10-01), 151-160 0014-2336 97:2<151 1997 97 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer