caa a22 4500 477134726 CHVBK 20180405111714.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1003026125623 doi (NATIONALLICENCE)springer-10.1023/A:1003026125623 Production of somatic hybrids between S. tuberosum L. and late blight resistant Mexican wild potato species [Elektronische Daten] [Ramona Thieme, Ulrich Darsow, Tatyana Gavrilenko, Dimitriy Dorokhov, Horst Tiemann] Interspecific somatic hybrids between dihaploid breeding clones of potato, S. tuberosum and two accessions of wild Mexican species S. pinnatisectum and the hybrid line S. pinnatisectum × S. bulbocastanum were regenerated following electrofusion of mesophyll protoplasts to combine important agricultural traits of S. tuberosum and a high level of late blight resistance from selected wild accessions. In two fusion combinations 239 calli were regenerated; 162 from 195 calli analysed were identified as hybrids by means of isozyme analysis of peroxidases and, for some hybrid clones, by RAPD analysis. Depending on the fusion combination, 47-89 percent of the somatic hybrids had the expected ploidy level and 7-16 percent were mixoploids. Somatic hybrids were phenotypically intermediate as compared to their parents and some of them were able to be backcrossed sexually with potato. Fertility and crossability depended on combination and ploidy level of the somatic hybrids. In tests with detached leaves the wild partner clones had a high late blight resistance score of 8,6 and 8,9; the susceptible tuberosum-partners of 2,8 and 3,5, respectively. Nearly 25 percent of somatic hybrids had a resistance level of 6 or higher in the first year of assessment. The average resistance value of most somatic hybrids was lower than the average parental level. The reasons for variation in resistance values are discussed in connection with the practical application of fusion hybrids. Kluwer Academic Publishers, 1997 protoplast fusion nationallicence resistance to Phytophthora infestans nationallicence Solanum bulbocastanum nationallicence Solanum pinnatisectum nationallicence Solanum tuberosum nationallicence Thieme Ramona Institute for Breeding of Crop Plants, Federal Centre for Breeding Research on Cultivated Plants, 18190, Groß Lüsewitz, Germany aut Darsow Ulrich Institute for Breeding of Crop Plants, Federal Centre for Breeding Research on Cultivated Plants, 18190, Groß Lüsewitz, Germany aut Gavrilenko Tatyana Laboratory of Biotechnology, N. I. Vavilov Institute of Plant Industry, B. Morskaya Str. 42, 190 000, St. Petersburg, Russia aut Dorokhov Dimitriy All-Russian Research Institute of Vegetable Breeding and Seed Production - VNIISSOK, Moscow, Russia aut Tiemann Horst Institute for Breeding of Crop Plants, Federal Centre for Breeding Research on Cultivated Plants, 18190, Groß Lüsewitz, Germany aut Euphytica Kluwer Academic Publishers 97/2(1997-10-01), 189-200 0014-2336 97:2<189 1997 97 10681 https://doi.org/10.1023/A:1003026125623 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003026125623 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thieme Ramona Institute for Breeding of Crop Plants, Federal Centre for Breeding Research on Cultivated Plants, 18190, Groß Lüsewitz, Germany aut NATIONALLICENCE 700 1- Darsow Ulrich Institute for Breeding of Crop Plants, Federal Centre for Breeding Research on Cultivated Plants, 18190, Groß Lüsewitz, Germany aut NATIONALLICENCE 700 1- Gavrilenko Tatyana Laboratory of Biotechnology, N. I. Vavilov Institute of Plant Industry, B. Morskaya Str. 42, 190 000, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Dorokhov Dimitriy All-Russian Research Institute of Vegetable Breeding and Seed Production - VNIISSOK, Moscow, Russia aut NATIONALLICENCE 700 1- Tiemann Horst Institute for Breeding of Crop Plants, Federal Centre for Breeding Research on Cultivated Plants, 18190, Groß Lüsewitz, Germany aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 97/2(1997-10-01), 189-200 0014-2336 97:2<189 1997 97 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer