caa a22 4500 477134734 CHVBK 20180405111714.0 cr unu---uuuuu 170330e19970701xx s 000 0 eng 10.1023/A:1002922016294 doi (NATIONALLICENCE)springer-10.1023/A:1002922016294 Molecular breeding in the genus Musa: a strong case for STMS marker technology [Elektronische Daten] [D. Kaemmer, D. Fischer, R.L. Jarret, F.-C. Baurens, A. Grapin, D. Dambier, J.-L. Noyer, C. Lanaud, G. Kahl, P.J.L. Lagoda] Musa species are among the tallest monocotyledons and include major food-producing species. The principal cultivars, derived from two major species Musa acuminata (‘A' genome) and Musa balbisiana (‘B' genome), are polyploid hybrids (mainly AAA, AAB and ABB triploids), medium to highly sterile, parthenocarpic and clonally propagated. Bananas and plantains are crops to which molecular breeding is expected to have a positive impact. In order to better understand banana genetics, more knowledge has to be accumulated about the complex genome structure of hybrids and cultivars. Therefore, the aim of our work is to develop molecular markers that are codominant, reliable, universal, highly polymorphic and that are applicable to collaborative Musa germplasm genotyping and mapping. Two size-selected genomic libraries have been screened for the presence of simple sequence repeats (SSR). Our data demonstrate that SSR are readily applicable to the study of Musa genetics. Our comprehensive analyses of a significant number of banana sequence tagged microsatellite sites (STMS) will add to our knowledge on the structure and phylogeny of genomes of the Musa species, and suggest that microsatellites be used as anchor markers for a banana genetic core map. Additional markers, such as e.g. CAPS have also been tested in order to increase the detection of polymorphisms exceeding that revealed by STMS technology. The utility of PCR-derived markers for collaborative genetic analyses of the banana genome, and the transferability of 'streamlined' laboratory techniques and data analysis to Developing Countries are discussed. Kluwer Academic Publishers, 1997 biotechnology nationallicence Musa nationallicence banana nationallicence plantain nationallicence germplasm genotyping nationallicence mapping nationallicence molecular breeding nationallicence PCR nationallicence microsatellites nationallicence Kaemmer D. Plant Molecular Biology, Biozentrum, Johann Wolfgang Goethe-University, Marie-Curie-Str. 9, D-60439, Frankfurt am Main, Germany aut Fischer D. Plant Molecular Biology, Biozentrum, Johann Wolfgang Goethe-University, Marie-Curie-Str. 9, D-60439, Frankfurt am Main, Germany aut Jarret R.L. Usda/ars, Plant Genetic Resources, 1109 Experiment Street, 30223, Griffin, Georgia, USA aut Baurens F.-C Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France aut Grapin A. Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France aut Dambier D. Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France aut Noyer J.-L Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France aut Lanaud C. Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France aut Kahl G. Plant Molecular Biology, Biozentrum, Johann Wolfgang Goethe-University, Marie-Curie-Str. 9, D-60439, Frankfurt am Main, Germany aut Lagoda P.J.L. Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France aut Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 49-63 0014-2336 96:1<49 1997 96 10681 https://doi.org/10.1023/A:1002922016294 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002922016294 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kaemmer D. Plant Molecular Biology, Biozentrum, Johann Wolfgang Goethe-University, Marie-Curie-Str. 9, D-60439, Frankfurt am Main, Germany aut NATIONALLICENCE 700 1- Fischer D. Plant Molecular Biology, Biozentrum, Johann Wolfgang Goethe-University, Marie-Curie-Str. 9, D-60439, Frankfurt am Main, Germany aut NATIONALLICENCE 700 1- Jarret R.L. Usda/ars, Plant Genetic Resources, 1109 Experiment Street, 30223, Griffin, Georgia, USA aut NATIONALLICENCE 700 1- Baurens F.-C Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France aut NATIONALLICENCE 700 1- Grapin A. Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France aut NATIONALLICENCE 700 1- Dambier D. Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France aut NATIONALLICENCE 700 1- Noyer J.-L Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France aut NATIONALLICENCE 700 1- Lanaud C. Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France aut NATIONALLICENCE 700 1- Kahl G. Plant Molecular Biology, Biozentrum, Johann Wolfgang Goethe-University, Marie-Curie-Str. 9, D-60439, Frankfurt am Main, Germany aut NATIONALLICENCE 700 1- Lagoda P.J.L. Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 49-63 0014-2336 96:1<49 1997 96 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer