caa a22 4500 477134769 CHVBK 20180405111714.0 cr unu---uuuuu 170330e19970701xx s 000 0 eng 10.1023/A:1002926421430 doi (NATIONALLICENCE)springer-10.1023/A:1002926421430 Use of somaclonal variation and in vitro selection for chilling tolerance improvement in rice [Elektronische Daten] [Pierre Bertin, Jules Bouharmont] Embryo-derived calli of four rice varieties cultivated at high altitude in Burundi — Facagro 57, Facagro 76, Kirundo 3 and Kirundo 9 — were submitted to different temperature regimes. The percentage of regenerating calli greatly varied depending on variety, length of culture and callus temperature treatment. The reduction of regeneration percentages induced by low temperature was more pronounced in the more sensitive varieties. Regenerated plants (R0) and their progenies in R1, R2 and R3 were cold-screened together with control plants. In all varieties, significantly higher survival rates were obtained in R3 with in vitro plants than with control plants. Such chilling tolerance improvement was not obtained following a massal selection applied during 3 successive generations onto the control plants. In vitro plants regenerated from calli cultivated either at 25 °C, either at 4 °C, were cultivated at different altitudes in Burundi during two successive generations. For most observed traits, the in vitro plants were characterized by lower means, larger variation and higher maximum values than the control plants. The most chilling-tolerant somaclonal families were most usually characterized by extensive differences in fatty acid composition, chilling-induced electrolyte leakage and chlorophyll fluorescence, compared to the varieties they derived from. Kluwer Academic Publishers, 1997 Oryza sativa nationallicence chilling tolerance nationallicence in vitro selection nationallicence rice nationallicence somaclonal variation nationallicence field performance nationallicence Bertin Pierre Laboratoire de Cytogénétique, Université Catholique de Louvain, Place Croix du Sud, 5 b13, B-1348, Louvain-la-Neuve, Belgium aut Bouharmont Jules Laboratoire de Cytogénétique, Université Catholique de Louvain, Place Croix du Sud, 5 b13, B-1348, Louvain-la-Neuve, Belgium aut Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 135-142 0014-2336 96:1<135 1997 96 10681 https://doi.org/10.1023/A:1002926421430 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002926421430 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bertin Pierre Laboratoire de Cytogénétique, Université Catholique de Louvain, Place Croix du Sud, 5 b13, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Bouharmont Jules Laboratoire de Cytogénétique, Université Catholique de Louvain, Place Croix du Sud, 5 b13, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 135-142 0014-2336 96:1<135 1997 96 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer