caa a22 4500 477134793 CHVBK 20180405111714.0 cr unu---uuuuu 170330e19970701xx s 000 0 eng 10.1023/A:1002997319342 doi (NATIONALLICENCE)springer-10.1023/A:1002997319342 Plant regeneration in sweet potato (Ipomoea batatas L., Convolvulaceae) [Elektronische Daten] [D. Sihachakr, R. Haïcour, J.M. Cavalcante Alves, I. Umboh, D. Nzoghé, A. Servaes, G. Ducreux] The application of new techniques for improvement of sweet potato crops, particularly including the exploitation of somaclonal variation, gene transfer by genetic transformation and somatic hybridization, requires the control of plant regeneration from tissue cultures. Shoots can easily be regenerated from explants of stems, petioles, leaves and roots, while callus cultures do not produce any shoots. The potential of somatic embryogenesis and plant regeneration via embryogenesis was evaluated for 10 cultivars of sweet potato. Protocols for plant regeneration from cultured protoplasts have also been developed. Since mesophyll was resistant to enzyme digestion, fragments of stems and petioles, callus and cell suspensions were used as source of protoplasts of sweet potato. Series of transfers of protoplast-derived calluses, particularly those which had been obtained from in vitro plants, to media containing a high level of zeatin resulted in successful formation of shoots in only two sweet potato cultivars. In addition, the embryogenic potential was irreversibly lost through protoplast culture, since protoplasts isolated from embryogenic cell suspensions developed into non-embryogenic callus. Consequently, an alternative protocol is being successfully developed to improve plant regeneration from cultured protoplasts of sweet potato, involving first root formation from which shoots can then be regenerated. Preliminary evaluation in field conditions in Gabon revealed that plants regenerated from cultured protoplasts exhibited a great genetic variability in their growth and tuber formation in particular. Kluwer Academic Publishers, 1997 Sweet potato nationallicence Ipomoea batatas nationallicence plant regeneration nationallicence somatic embryogenesis nationallicence protoplasts nationallicence genetic variation nationallicence somaclonal variation nationallicence Sihachakr D. Morphogenèse Végétale Expérimentale, Université Paris Sud, Bât, 360, 91405, Orsay Cedex, France aut Haïcour R. Morphogenèse Végétale Expérimentale, Université Paris Sud, Bât, 360, 91405, Orsay Cedex, France aut Cavalcante Alves J.M. Morphogenèse Végétale Expérimentale, Université Paris Sud, Bât, 360, 91405, Orsay Cedex, France aut Umboh I. Seameo - Biotrop, Jl Raya Tajur Km 6, P.O. Box 116, Bogor, Indonesia aut Nzoghé D. Projet FAO - CIAM, Laboratoire de culture in vitro, BP 2183, Libreville, Gabon aut Servaes A. Morphogenèse Végétale Expérimentale, Université Paris Sud, Bât, 360, 91405, Orsay Cedex, France aut Ducreux G. Morphogenèse Végétale Expérimentale, Université Paris Sud, Bât, 360, 91405, Orsay Cedex, France aut Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 143-152 0014-2336 96:1<143 1997 96 10681 https://doi.org/10.1023/A:1002997319342 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002997319342 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sihachakr D. Morphogenèse Végétale Expérimentale, Université Paris Sud, Bât, 360, 91405, Orsay Cedex, France aut NATIONALLICENCE 700 1- Haïcour R. Morphogenèse Végétale Expérimentale, Université Paris Sud, Bât, 360, 91405, Orsay Cedex, France aut NATIONALLICENCE 700 1- Cavalcante Alves J.M. Morphogenèse Végétale Expérimentale, Université Paris Sud, Bât, 360, 91405, Orsay Cedex, France aut NATIONALLICENCE 700 1- Umboh I. Seameo - Biotrop, Jl Raya Tajur Km 6, P.O. Box 116, Bogor, Indonesia aut NATIONALLICENCE 700 1- Nzoghé D. Projet FAO - CIAM, Laboratoire de culture in vitro, BP 2183, Libreville, Gabon aut NATIONALLICENCE 700 1- Servaes A. Morphogenèse Végétale Expérimentale, Université Paris Sud, Bât, 360, 91405, Orsay Cedex, France aut NATIONALLICENCE 700 1- Ducreux G. Morphogenèse Végétale Expérimentale, Université Paris Sud, Bât, 360, 91405, Orsay Cedex, France aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 143-152 0014-2336 96:1<143 1997 96 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer