caa a22 4500 477134823 CHVBK 20180405111714.0 cr unu---uuuuu 170330e19970701xx s 000 0 eng 10.1023/A:1002970925897 doi (NATIONALLICENCE)springer-10.1023/A:1002970925897 Regeneration and transformation of cassava [Elektronische Daten] [C.J.J.M. Raemakers, E. Sofiari, E. Jacobsen, R.G.F. Visser] A prerequisite for the development of a successful transformation system is the availability of efficient regeneration systems. Up to 1995 the only available regeneration system in cassava was an organized type of somatic embryogenesis. Transformation of these organized somatic embryogenic cultures with particle bombardment or Agrobacterium tumefaciens resulted in chimeric transformed embryos. However, the transformed sector was lost after repeated cycles of secondary somatic embryogenesis. After 1995 a less organized system of somatic embryogenesis was developed, so called friable embryogenic callus (FEC) and a system of adventitious shoot regeneration. The FEC regeneration system was combined successfully with particle bombardment. Selection of transgenic plants was based on either luciferase activity, or resistance to the aminoglycoside paromomycin or the herbicide phosphinothricin. Furthermore, protoplasts of FEC are able to regenerate into plants and can be transformed by electroporation. The adventitious shoot regeneration system was combined successfully with Agrobacterium tumefaciens. For this mature somatic embryos were cocultivated with Agrobacterium and cultured for adventitious shoot development. After selection based on the aminoglycoside geneticin or on hygromycin transgenic plants were formed. Kluwer Academic Publishers, 1997 cassava nationallicence transformation nationallicence review nationallicence somatic embryogenesis nationallicence adventitious shoot formation nationallicence Raemakers C.J.J.M. The Graduate School Experimental Plant Sciences, Department of Plant Breeding, Agricultural University Wageningen, P.O.Box 386, 6700 AJ, Wageningen, The Netherlands aut Sofiari E. The Graduate School Experimental Plant Sciences, Department of Plant Breeding, Agricultural University Wageningen, P.O.Box 386, 6700 AJ, Wageningen, The Netherlands aut Jacobsen E. The Graduate School Experimental Plant Sciences, Department of Plant Breeding, Agricultural University Wageningen, P.O.Box 386, 6700 AJ, Wageningen, The Netherlands aut Visser R.G.F. The Graduate School Experimental Plant Sciences, Department of Plant Breeding, Agricultural University Wageningen, P.O.Box 386, 6700 AJ, Wageningen, The Netherlands aut Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 153-161 0014-2336 96:1<153 1997 96 10681 https://doi.org/10.1023/A:1002970925897 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002970925897 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Raemakers C.J.J.M. The Graduate School Experimental Plant Sciences, Department of Plant Breeding, Agricultural University Wageningen, P.O.Box 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Sofiari E. The Graduate School Experimental Plant Sciences, Department of Plant Breeding, Agricultural University Wageningen, P.O.Box 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Jacobsen E. The Graduate School Experimental Plant Sciences, Department of Plant Breeding, Agricultural University Wageningen, P.O.Box 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Visser R.G.F. The Graduate School Experimental Plant Sciences, Department of Plant Breeding, Agricultural University Wageningen, P.O.Box 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 153-161 0014-2336 96:1<153 1997 96 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer