caa a22 4500 477134831 CHVBK 20180405111714.0 cr unu---uuuuu 170330e19970701xx s 000 0 eng 10.1023/A:1002950300536 doi (NATIONALLICENCE)springer-10.1023/A:1002950300536 Towards a latex molecular diagnostic of yield potential and the genetic engineering of the rubber tree [Elektronische Daten] [H. Chrestin, X. Gidrol, A. Kush] The latex from Hevea brasiliensis is expelled from specialized cells upon bark tapping. The latex yield is mainly limited by the duration of the latex flow, which is controlled by coagulation processes. Bark treatment with ethylene is known to delay coagulation and increase latex yield. The molecular basis of latex coagulation has been characterized: (1) Hevein, a lectin-like protein, induces latex coagulation by bringing together the rubber particles (RPs). The hevein-RPs bridging is mediated by N-Acetyl-D-glucosamine, and involves a 22 kDa receptor glycoprotein localized on the RPs surface. This process is inhibited by the removal of the sugar moiety from the receptor, through the action of N-acetyl-glucosaminidase and chitinases. (2) Ethylene induces, in the latex cells, an over-expression of the 3 genes coding for hevein and its receptor, and a chitinase. The higher over-expression of one chitinase can explain the partial deglycosylation of the hevein receptor and the resulting delay in coagulation. (3) The level of hevein and chitinase expression in the latex is a clonal characteristic, linked to the characteristics of the latex flow. Expression of these genes might be used as molecular markers for high yield potential. Based on these findings, it would be interesting to improve the rubber tree through the genetic engineering technics, to get new high yielding cultivars with prolonged latex flow. Kluwer Academic Publishers, 1997 ethylene nationallicence gene expression nationallicence Hevea brasiliensis nationallicence latex coagulation nationallicence rubber nationallicence somatic embryogenesis nationallicence Chrestin H. Orstom-Cirad Institute (France/Thailand)/Dept. of Biotechnology, Mahidol University, Faculty of Science, Rama VI road, 10400, Bangkok, Thailand aut Gidrol X. Orstom-Cirad Institute (France/Thailand)/Dept. of Biotechnology, Mahidol University, Faculty of Science, Rama VI road, 10400, Bangkok, Thailand aut Kush A. Imcb/nus, 10 Kent Ridge Crescent, Singapore aut Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 77-82 0014-2336 96:1<77 1997 96 10681 https://doi.org/10.1023/A:1002950300536 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002950300536 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chrestin H. Orstom-Cirad Institute (France/Thailand)/Dept. of Biotechnology, Mahidol University, Faculty of Science, Rama VI road, 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Gidrol X. Orstom-Cirad Institute (France/Thailand)/Dept. of Biotechnology, Mahidol University, Faculty of Science, Rama VI road, 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Kush A. Imcb/nus, 10 Kent Ridge Crescent, Singapore aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 77-82 0014-2336 96:1<77 1997 96 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer