caa a22 4500 477134858 CHVBK 20180405111715.0 cr unu---uuuuu 170330e19970701xx s 000 0 eng 10.1023/A:1003045518236 doi (NATIONALLICENCE)springer-10.1023/A:1003045518236 First steps towards a rational use of African rice, Oryza glaberrima, in rice breeding through a ‘contig line' concept [Elektronische Daten] [A. Ghesquière, J. Séquier, G. Second, M. Lorieux] In the past, utilisation of African cultivated rice, Oryza glaberrima, in O. sativa breeding was hampered by high sterility in interspecific F1 and in early progenies. Recent development of mapped molecular markers provides the opportunity to rationalise and to monitor introgressions between the two cultivated rice species. Development of such introgressed lines through interspecific hybridisation and backcrossing represents high potential to create new genetic and transgressive variation. We are initiating an original marker-aided backcross program with the aim of producing a set of 100 ‘contig lines', each one bearing an alien O. glaberrima chromosomal fragment of around 20 cM in the O. sativa genetic background. As a preliminary step, we are now developing a genetic linkage map based on STS and RAPD markers, using an interspecific backcross between O. sativa and O. glaberrima. BC1 plants will then be selected to expedite the production of ‘contig lines' exhibiting only the target fragment. The interspecific polymorphism is anticipated to be large enough to make this methodology feasible. Many advantages are expected from this approach, such as: (i) systematic assessment of the useful genetic resources displayed by O. glaberrima, (ii) improved and simplified detection of QTLs and (iii) comparative analysis of genomic organisation of O. glaberrima vs. O. sativa. Current work on evaluation of STS and RAPD polymorphism between parents and development of the BC1 population is presented. Kluwer Academic Publishers, 1997 ‘contig lines' nationallicence interspecific hybridisation nationallicence genetic resources nationallicence molecular markers nationallicence Oryza glaberrima nationallicence Oryza sativa nationallicence Ghesquière A. Orstom-Lrgapt, Bp 5045, 34032, Montpellier Cedex 1, France aut Séquier J. Orstom-Lrgapt, Bp 5045, 34032, Montpellier Cedex 1, France aut Second G. Orstom-Lrgapt, Bp 5045, 34032, Montpellier Cedex 1, France aut Lorieux M. Orstom-Lrgapt, Bp 5045, 34032, Montpellier Cedex 1, France aut Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 31-39 0014-2336 96:1<31 1997 96 10681 https://doi.org/10.1023/A:1003045518236 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003045518236 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ghesquière A. Orstom-Lrgapt, Bp 5045, 34032, Montpellier Cedex 1, France aut NATIONALLICENCE 700 1- Séquier J. Orstom-Lrgapt, Bp 5045, 34032, Montpellier Cedex 1, France aut NATIONALLICENCE 700 1- Second G. Orstom-Lrgapt, Bp 5045, 34032, Montpellier Cedex 1, France aut NATIONALLICENCE 700 1- Lorieux M. Orstom-Lrgapt, Bp 5045, 34032, Montpellier Cedex 1, France aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 31-39 0014-2336 96:1<31 1997 96 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer