caa a22 4500 477134920 CHVBK 20180405111715.0 cr unu---uuuuu 170330e19970701xx s 000 0 eng 10.1023/A:1002906308304 doi (NATIONALLICENCE)springer-10.1023/A:1002906308304 Introduction of new traits into cotton through genetic engineering: insect resistance as example [Elektronische Daten] [C. Pannetier, M. Giband, P. Couzi, V. Le Tan, M. Mazier, J. Tourneur, B. Hau] The main goal of gene transfer into cotton is the development of insect-resistant varieties. The stakes are important since cotton protection against insects uses almost 24% of the world's chemical insecticides market, which is not without consequences on the environment. The first approach was to introduce and express in the cotton genome, genes from the bacterium Bacillus thuringiensis (B.t.) which produces entomopathogenic toxins. The development of an efficient Agrobacterium tumefaciens mediated transformation system was the first step. The expression of B.t. genes was studied and synthetic genes more adapted to a plant genome have been constructed. Studies on their expression in cotton is underway. The second focus was to develop strategies that would minimize the risks of inducing insect resistance. The main approach is to associate several genes coding for entomopathogenic proteins with different modes of action. Genes encoding protease inhibitors were chosen. One possibility is to associate a B.t. gene and a gene encoding a protease inhibitor. Several protease inhibitors were tested in artificial diets on major pests of cotton. The corresponding genes have been introduced into the cotton genome. These various orientations of the research program will be presented. Kluwer Academic Publishers, 1997 Agrobacterium tumefaciens nationallicence Bacillus thuringiensis nationallicence cotton nationallicence gene transfer nationallicence Gossypium hirsutum nationallicence insect resistance nationallicence protease inhibitors nationallicence regeneration nationallicence Pannetier C. Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, 78026, Versailles Cedex, France aut Giband M. Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, 78026, Versailles Cedex, France aut Couzi P. Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, 78026, Versailles Cedex, France aut Le Tan V. Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, 78026, Versailles Cedex, France aut Mazier M. Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, 78026, Versailles Cedex, France aut Tourneur J. Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, 78026, Versailles Cedex, France aut Hau B. Cirad/ca, Bp 5035, 34032, Montpellier Cedex, France aut Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 163-166 0014-2336 96:1<163 1997 96 10681 https://doi.org/10.1023/A:1002906308304 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002906308304 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pannetier C. Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Giband M. Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Couzi P. Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Le Tan V. Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Mazier M. Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Tourneur J. Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Hau B. Cirad/ca, Bp 5035, 34032, Montpellier Cedex, France aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 96/1(1997-07-01), 163-166 0014-2336 96:1<163 1997 96 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer