caa a22 4500 477134963 CHVBK 20180405111715.0 cr unu---uuuuu 170330e19970801xx s 000 0 eng 10.1023/A:1003061501477 doi (NATIONALLICENCE)springer-10.1023/A:1003061501477 Direct comparison of pollen-mediated movement of native and engineered genes [Elektronische Daten] [Stan Hokanson, James Hancock, Rebecca Grumet] Despite the commercial approval of twenty-five transgenic crops in the U.S. as of mid-1996, concern is still being expressed regarding the potential risks associated with genetically engineered crops. One recurring issue is the possibility of pollen-mediated escape of engineered genes into populations of crop wild relatives. To address this concern, the scientific community has depended on literature on pollen dispersal generated from non-transgenic organisms. Utilization of this information requires the assumption that the pollen mediated movement of native and transgenes is the same. To test the validity of this assumption, we directly compared the pollen-mediated gene movement of native and engineered marker genes using melon plants (Cucumis melo L.) expressing dominant morphological and transgenic traits. Movement into both contiguous border plots and non-contiguous satellite plots were monitored. Dispersal of the native gene and transgene into the satellite plots was identical. Dispersal of the two traits into the plot borders was nearly identical. Of the nearly 4600 seedlings screened for both morphological (presence of green vs. virescent cotyledons) and transgene movement (presence of NPT II protein by ELISA), in no case was the NPT II gene observed in the absence of green cotyledons. However, 39 seedlings were green but did not express NPT II as measured by ELISA. PCR analysis revealed transgene inactivation as a cause of the NPT II ELISA-seedlings. Kluwer Academic Publishers, 1997 Cucumis melo nationallicence gene movement nationallicence gene silencing nationallicence pollen flow nationallicence risk assessment nationallicence transgenic plants nationallicence Hokanson Stan Department of Horticulture, Michigan State University, 48824, East Lansing, Mi, USA aut Hancock James Department of Horticulture, Michigan State University, 48824, East Lansing, Mi, USA aut Grumet Rebecca Department of Horticulture, Michigan State University, 48824, East Lansing, Mi, USA aut Euphytica Kluwer Academic Publishers 96/3(1997-08-01), 397-403 0014-2336 96:3<397 1997 96 10681 https://doi.org/10.1023/A:1003061501477 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003061501477 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hokanson Stan Department of Horticulture, Michigan State University, 48824, East Lansing, Mi, USA aut NATIONALLICENCE 700 1- Hancock James Department of Horticulture, Michigan State University, 48824, East Lansing, Mi, USA aut NATIONALLICENCE 700 1- Grumet Rebecca Department of Horticulture, Michigan State University, 48824, East Lansing, Mi, USA aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 96/3(1997-08-01), 397-403 0014-2336 96:3<397 1997 96 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer