caa a22 4500 477135013 CHVBK 20180405111715.0 cr unu---uuuuu 170330e19970801xx s 000 0 eng 10.1023/A:1003045000568 doi (NATIONALLICENCE)springer-10.1023/A:1003045000568 The use of Rapd markers for lentil genetic mapping and the evaluation of distorted F2 segregation [Elektronische Daten] [I. Eujayl, M. Baum, W. Erskine, E. Pehu, F.J. Muehlbauer] To maximize the extent of polymorphism within a mapping population wide crosses are often made, frequently resulting in distorted segregation. Two parents used in the crosses in this study contained ca 50% from wild lentil genome (Lens culinaris ssp. Orientalis). We investigated the use of random amplified polymorphic DNA (RAPD) in the lentil (Lens culinaris Med.), for genetic mapping and testing for segregation distortion in F2 populations. In cross 1, 83% of the RAPD markers showed segregation distortion, which was also observed for isozyme and morphological loci. By contrast, in cross 2, there was little (10%) segregation distortion. Out of 390 primers tested, 116 primers (29.7%) yielded 192 polymorphic fragments between parents of cross 2. This polymorphism was confirmed as reproducible. Seventy-eight segregating loci were analyzed for linkage, at a LOD score > 3.0, resulted in 28 RAPD, one RFLP, one morphological and three oligonucleotide markers, which were assigned to 9 linkage groups spanning 206 cM. Clearly, in lentil RAPD markers were valuable for genetic mapping and evaluation of segregation distortion. Kluwer Academic Publishers, 1997 Lens culinaris nationallicence linkage nationallicence RAPD nationallicence segregation distortion nationallicence Eujayl I. Icarda, P.O. Box 5466, Aleppo, Syria aut Baum M. Icarda, P.O. Box 5466, Aleppo, Syria aut Erskine W. Icarda, P.O. Box 5466, Aleppo, Syria aut Pehu E. Department of Plant Production, Pl 27 (viikki, C-talo), University of Helsinki, 00014, Finland aut Muehlbauer F.J. Grain Legume Genetics and Physiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Washington State University, Pullman, Washington, USA aut Euphytica Kluwer Academic Publishers 96/3(1997-08-01), 405-412 0014-2336 96:3<405 1997 96 10681 https://doi.org/10.1023/A:1003045000568 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003045000568 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Eujayl I. Icarda, P.O. Box 5466, Aleppo, Syria aut NATIONALLICENCE 700 1- Baum M. Icarda, P.O. Box 5466, Aleppo, Syria aut NATIONALLICENCE 700 1- Erskine W. Icarda, P.O. Box 5466, Aleppo, Syria aut NATIONALLICENCE 700 1- Pehu E. Department of Plant Production, Pl 27 (viikki, C-talo), University of Helsinki, 00014, Finland aut NATIONALLICENCE 700 1- Muehlbauer F.J. Grain Legume Genetics and Physiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Washington State University, Pullman, Washington, USA aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 96/3(1997-08-01), 405-412 0014-2336 96:3<405 1997 96 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer