caa a22 4500 477135099 CHVBK 20180405111715.0 cr unu---uuuuu 170330e19970801xx s 000 0 eng 10.1023/A:1003038419126 doi (NATIONALLICENCE)springer-10.1023/A:1003038419126 Somatic hybridization between Solanum tuberosum and species of the S. nigrum complex: Selection of vigorously growing and flowering plants [Elektronische Daten] [K. Horsman, J.E.M. Bergervoet, E. Jacobsen] Fusion experiments were performed between diploid (2n = 2x = 24) or tetraploid (2n = 4x = 48) potato genotypes and four species of the Solanum nigrum complex, namely S. nigrum (2n = 6x = 72), S. villosum (2n = 4x = 48), S. chenopodioides (2n = 2x = 24) or S. americanum (2n = 2x = 24 and 2n = 6x = 72). All five accessions of the S. nigrum-species were successfully hybridized with at least one of the potato genotypes. Somatic combining abilities were influenced by the ploidy level as well as the genotype of the parental species. The use of kanamycin or hygromycin resistance as cell-selectable markersystem had no influence on somatic combining ability, but such markers can be useful to improve efficient selection of somatic hybrids in sufficient numbers. At least 20% of the hybrids of each successful combination performed well in vitro. However, only 60 genotypes out of 761 somatic hybrids were vigorous as well as flowering in the greenhouse. Analysis of the DNA content of somatic hybrids could be used as a criterion for the indirect selection in vitro of hybrids that were vigorous in the greenhouse. Flowering somatic hybrids of S. nigrum (+) 2x potato and S. americanum (+) 4x potato were selected with the aim of introgression of resistance traits after recurrent backcrossing with cultivated potato. Kluwer Academic Publishers, 1997 potato nationallicence Solanum nigrum complex nationallicence somatic hybridization nationallicence hybrid selection criterions nationallicence cell-selectable markers nationallicence DNA content nationallicence Horsman K. The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 Aj, Wageningen, the Netherlands aut Bergervoet J.E.M. The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 Aj, Wageningen, the Netherlands aut Jacobsen E. The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 Aj, Wageningen, the Netherlands aut Euphytica Kluwer Academic Publishers 96/3(1997-08-01), 345-352 0014-2336 96:3<345 1997 96 10681 https://doi.org/10.1023/A:1003038419126 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003038419126 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Horsman K. The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 Aj, Wageningen, the Netherlands aut NATIONALLICENCE 700 1- Bergervoet J.E.M. The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 Aj, Wageningen, the Netherlands aut NATIONALLICENCE 700 1- Jacobsen E. The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 Aj, Wageningen, the Netherlands aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 96/3(1997-08-01), 345-352 0014-2336 96:3<345 1997 96 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer