caa a22 4500 477135153 CHVBK 20180405111715.0 cr unu---uuuuu 170330e19970701xx s 000 0 eng 10.1023/A:1003097600701 doi (NATIONALLICENCE)springer-10.1023/A:1003097600701 Diversity analysis and species identification in Lens using PCR generated markers [Elektronische Daten] [R. Ford, E.C.K. Pang, P.W.J. Taylor] Using random amplified polymorphic DNA (RAPD) analysis we assessed the genetic relationships between 16 accessions and cultivars of lentil (Lens culinaris ssp. culinaris) in the Australian lentil breeding program. All lines exhibited polymorphism with a maximum dissimilarity value of 0.36. This indicated a limited degree of genetic variation. Polymerase chain reaction (PCR) with primers based on the flanking regions of the 5S rRNA gene from Pisum sativum amplified the non-translated spacer (NTS) region from within the 5S rRNA gene of Lens. Three distinct amplification banding patterns differentiated between restricted genomic DNA of Lens spp. L. culinaris ssp. culinaris and L. culinaris ssp. orientalis shared similar markers of two distinctly different NTS sizes. L. nigricans and L. odemensis shared the same amplification pattern of a single sized NTS region. However, L. ervoides contained two separate sizes of NTS, distinct from other Lens species. In an effort to widen the genetic base of cultivated lentil, these species-specific molecular markers may be used to follow potential introgression between species. Kluwer Academic Publishers, 1997 RAPD nationallicence PCR nationallicence genetic diversity nationallicence 5S rRNA nationallicence Lens nationallicence lentil nationallicence Ford R. Molecular Marker Laboratory, Joint Centre for Crop Improvement, Department of Agriculture and Resource Management, University of Melbourne, 3054, Parkville, Victoria, Australia aut Pang E.C.K. Molecular Marker Laboratory, Joint Centre for Crop Improvement, Department of Agriculture and Resource Management, University of Melbourne, 3054, Parkville, Victoria, Australia aut Taylor P.W.J. Molecular Marker Laboratory, Joint Centre for Crop Improvement, Department of Agriculture and Resource Management, University of Melbourne, 3054, Parkville, Victoria, Australia aut Euphytica Kluwer Academic Publishers 96/2(1997-07-01), 247-255 0014-2336 96:2<247 1997 96 10681 https://doi.org/10.1023/A:1003097600701 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003097600701 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ford R. Molecular Marker Laboratory, Joint Centre for Crop Improvement, Department of Agriculture and Resource Management, University of Melbourne, 3054, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Pang E.C.K. Molecular Marker Laboratory, Joint Centre for Crop Improvement, Department of Agriculture and Resource Management, University of Melbourne, 3054, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Taylor P.W.J. Molecular Marker Laboratory, Joint Centre for Crop Improvement, Department of Agriculture and Resource Management, University of Melbourne, 3054, Parkville, Victoria, Australia aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 96/2(1997-07-01), 247-255 0014-2336 96:2<247 1997 96 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer