caa a22 4500 477135218 CHVBK 20180405111715.0 cr unu---uuuuu 170330e19970701xx s 000 0 eng 10.1023/A:1003034215722 doi (NATIONALLICENCE)springer-10.1023/A:1003034215722 Selection of cell-lines and regeneration of plants resistant to fusaric acid from Gladiolus × grandiflorus cv. ‘Peter Pears' [Elektronische Daten] [Patrizio Remotti, Hubertus Löffler, Lous van Vloten-Doting] Cell suspension cultures of ‘Peter Pears', a cultivar of Gladiolus x grandiflorus (Hort.), susceptible to the fungus Fusarium oxysporum f. sp. gladioli (Mass.), have been challenged with fusaric acid, one of the toxins produced by this pathogen. Selected cell-lines showed increased tolerance to the toxin and grew even on concentrations of fusaric acid up to 0.5 mm. When inoculated with a conidial suspension, the mycelial growth on selected cell-lines was limited compared to the control. Fusaric acid greatly reduced plant regeneration, and only two plants were obtained from the callus subcultured for a prolonged time on medium with fusaric acid. These plants have a significantly altered DNA content compared to the control. A reduced callus phase on toxin-containing medium, resulted in an improved regeneration and the DNA content of the regenerated plantlets was similar to the control. Some plants regenerated from these fusaric acid-insensitive cell-lines showed an increased tolerance to the toxin when cultured in vitro in presence of fusaric acid. The selected plants will be further tested for Fusarium-resistance once maturity is reached. Kluwer Academic Publishers, 1997 disease-resistance nationallicence Fusaric acid nationallicence Fusarium oxysporum f. sp. gladioli nationallicence Gladiolus nationallicence In vitro selection nationallicence Remotti Patrizio Department of Ornamental Crops, DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut Löffler Hubertus Department of Ornamental Crops, DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut van Vloten-Doting Lous Department of Experimental Botany, Catholic University Nijmegen, Toernooiveld 6525 ED, Nijmegen, The Netherlands aut Euphytica Kluwer Academic Publishers 96/2(1997-07-01), 237-245 0014-2336 96:2<237 1997 96 10681 https://doi.org/10.1023/A:1003034215722 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1003034215722 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Remotti Patrizio Department of Ornamental Crops, DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Löffler Hubertus Department of Ornamental Crops, DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- van Vloten-Doting Lous Department of Experimental Botany, Catholic University Nijmegen, Toernooiveld 6525 ED, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 96/2(1997-07-01), 237-245 0014-2336 96:2<237 1997 96 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer