caa a22 4500 477135498 CHVBK 20180405111716.0 cr unu---uuuuu 170330e19970601xx s 000 0 eng 10.1023/A:1002901112164 doi (NATIONALLICENCE)springer-10.1023/A:1002901112164 Genetic variation of mitochondrial and nuclear genomes in carrots revealed by random amplified polymorphic DNA (RAPD) [Elektronische Daten] [Yuki Nakajima, Toshiya Yamamoto, Kenji Oeda] Mitochondrial and nuclear genomic diversities of 8 carrot (Daucus carota ssp. sativus) varieties, including 6 pure lines and 2 cytoplasmic male sterile (cms) lines, were taxonomically identified using PCR with 19 RAPD primers. Dendrograms based on polymorphisms of both mitochondrial and nuclear genomes were constructed. According to the dendrogram of the mitochondrial genome revealed by RAPD, 4 differentiated clusters formed, in good accordance with the classification based on analyses with restriction enzyme digestion. Two cms lines were grouped into the same cluster, as genetically separated from the others. Thus, the cytoplasm donors of these male sterile lines were thought to be wild carrots. Conversely, RAPD analysis of the nuclear genome for these eight cultivars revealed no evident clusters although some cultivars were of a similar origin or place of cultivation. A correlation between nuclear and mitochondrial dendrograms was absent. RAPD has proved to be a useful tool for identifying mitochondrial and nuclear genomes. This technique will greatly aid in promoting efficient improvement of carrots. Kluwer Academic Publishers, 1997 cytoplasmic male sterility nationallicence Daucus carota nationallicence ssp. sativus nationallicence mitochondrial DNA nationallicence pedigree analysis nationallicence RAPD nationallicence Nakajima Yuki Biotechnology Laboratory, Sumitomo Chemical Co., Ltd., 4-2-1 Takatsukasa, 665, Takarazuka, Hyogo, Japan aut Yamamoto Toshiya Biotechnology Laboratory, Sumitomo Chemical Co., Ltd., 4-2-1 Takatsukasa, 665, Takarazuka, Hyogo, Japan aut Oeda Kenji Biotechnology Laboratory, Sumitomo Chemical Co., Ltd., 4-2-1 Takatsukasa, 665, Takarazuka, Hyogo, Japan aut Euphytica Kluwer Academic Publishers 95/3(1997-06-01), 259-267 0014-2336 95:3<259 1997 95 10681 https://doi.org/10.1023/A:1002901112164 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002901112164 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakajima Yuki Biotechnology Laboratory, Sumitomo Chemical Co., Ltd., 4-2-1 Takatsukasa, 665, Takarazuka, Hyogo, Japan aut NATIONALLICENCE 700 1- Yamamoto Toshiya Biotechnology Laboratory, Sumitomo Chemical Co., Ltd., 4-2-1 Takatsukasa, 665, Takarazuka, Hyogo, Japan aut NATIONALLICENCE 700 1- Oeda Kenji Biotechnology Laboratory, Sumitomo Chemical Co., Ltd., 4-2-1 Takatsukasa, 665, Takarazuka, Hyogo, Japan aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 95/3(1997-06-01), 259-267 0014-2336 95:3<259 1997 95 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer