caa a22 4500 477135595 CHVBK 20180405111716.0 cr unu---uuuuu 170330e19970501xx s 000 0 eng 10.1023/A:1002949700854 doi (NATIONALLICENCE)springer-10.1023/A:1002949700854 Production of interspecific F1 hybrids, BC1, BC2 and BC3 populations between Lycopersicon esculentum and two accessions of Lycopersicon peruvianum carrying new root-knot nematode resistance genes [Elektronische Daten] [Sami Doganlar, Anne Frary, Steven Tanksley] Two accessions of Lycopersicon peruvianum Mill. (PI270435, PI126443) carrying Mi-2 and Mi-3, respectively, new root-knot nematode resistance genes, were selected as the male parents for crosses with L. esculentum Mill. in order to produce interspecific hybrids. Crossability barriers between these two distantly related species were circumvented by ovule culture. A total of ten interspecific F1 hybrid plants were produced. The hybrid nature of the putative F1 plants was confirmed by a comparison of several morphological characteristics and a PCR-based assay. Eight of ten hybrid plants were backcrossed with L. esculentum to generate a total of 98 BC1 progeny. Two lines were advanced to the BC2 and BC3 levels. Based on these results, ovule culture was found to be an effective method for the production of novel interspecific F1 and BC1 plants. Kluwer Academic Publishers, 1997 crossability barriers nationallicence ovule culture nationallicence interspecific crosses nationallicence Lycopersicon esculentum nationallicence Lycopersicon peruvianum nationallicence Doganlar Sami Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853-1902, Ithaca, NY, U.S.A aut Frary Anne Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853-1902, Ithaca, NY, U.S.A aut Tanksley Steven Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853-1902, Ithaca, NY, U.S.A aut Euphytica Kluwer Academic Publishers 95/2(1997-05-01), 203-207 0014-2336 95:2<203 1997 95 10681 https://doi.org/10.1023/A:1002949700854 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002949700854 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Doganlar Sami Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853-1902, Ithaca, NY, U.S.A aut NATIONALLICENCE 700 1- Frary Anne Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853-1902, Ithaca, NY, U.S.A aut NATIONALLICENCE 700 1- Tanksley Steven Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853-1902, Ithaca, NY, U.S.A aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 95/2(1997-05-01), 203-207 0014-2336 95:2<203 1997 95 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer