caa a22 4500 47713565X CHVBK 20180405111717.0 cr unu---uuuuu 170330e19970501xx s 000 0 eng 10.1023/A:1002946618273 doi (NATIONALLICENCE)springer-10.1023/A:1002946618273 RFLP analysis of Hypericum perforatum L. somaclones and their progenies [Elektronische Daten] [Jana Halušková, Eva Čellárová] Three adjacent EcoRI fragments of the rDNA unit from Lycopersicon esculentum, eleven anonymous genomic and two anonymous cDNA clones from Brassica napus and three restriction endonucleases: BamHI, EcoRI and EcoRV were used for RFLP analysis of the genome of Hypericum perforatum L. A polymorphic band identified with EcoRI and two rDNA probes in five somaclones originated from the same genotype was detected in all progenies of two somaclones indicating the inheritance of the molecular changes. rDNA unit heterogeneity represented by two types of RFLP pattern revealed among somaclones and seed-derived control plants using EcoRV and two rDNA probes may indicate an alloploid origin of Hypericum perforatum. The occurrence of the identical RFLP patterns in some R0 somaclones and seed-derived plants and their progenies may be related to the apomictic mode of reproduction which is assumed to be prevalent in Hypericum perforatum. The differences in RFLP patterns of progenies when compared with the maternal plants (1 out of 10 progenies of one control plant and 1 out of 8 progenies of one somaclone) may indicate that some progenies have originated through sexual recombination. Kluwer Academic Publishers, 1997 apomixis nationallicence Hypericum perforatum nationallicence rDNA nationallicence heterogeneity nationallicence RFLP nationallicence progeny nationallicence somaclonal variation nationallicence Halušková Jana Department of Experimental Botany and Genetics, Faculty of Science, P.J. Šafárik University, Košice, Slovakia aut Čellárová Eva Department of Experimental Botany and Genetics, Faculty of Science, P.J. Šafárik University, Košice, Slovakia aut Euphytica Kluwer Academic Publishers 95/2(1997-05-01), 229-235 0014-2336 95:2<229 1997 95 10681 https://doi.org/10.1023/A:1002946618273 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002946618273 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Halušková Jana Department of Experimental Botany and Genetics, Faculty of Science, P.J. Šafárik University, Košice, Slovakia aut NATIONALLICENCE 700 1- Čellárová Eva Department of Experimental Botany and Genetics, Faculty of Science, P.J. Šafárik University, Košice, Slovakia aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 95/2(1997-05-01), 229-235 0014-2336 95:2<229 1997 95 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer