caa a22 4500 477135668 CHVBK 20180405111717.0 cr unu---uuuuu 170330e19970501xx s 000 0 eng 10.1023/A:1002945632713 doi (NATIONALLICENCE)springer-10.1023/A:1002945632713 Floral flavonoids and pH in Dendrobium orchid species and hybrids [Elektronische Daten] [Adelheid Kuehnle, David Lewis, Kenneth Markham, Kevin Mitchell, Kevin Davies, Brian Jordan] Anthocyanidins were identified in 28 Dendrobium species and hybrids selected for analysis based on colour and suitability in cut flower breeding. Flowers designated pink, red, maroon, orange, bronze, and brown in the trade were placed in RHS colour groups red-purple, purple-violet, violet on yellow, greyed-purple on yellow or yellow-orange, and brown. This colour range contained anthocyanins based on cyanidin, with peonidin occurring as a minor pigment. The colours of three blue genotypes, D. gouldii K280-6, D. biggibum ‘blue', and D. Kultana ‘blue', were light violet to purple by RHS standards and contained anthocyanins based on cyanidin. Peach-coloured flowers were classified as red or red-purple and included pelargonidin glycosides. Anthocyanin concentrations ranged from 0.13 to 0.18 μmoles/g FW in light lavender and peach, and up to 3.66 μmoles/g FW in brown. Combined cellular and vacuolar pH ranged narrowly from 4.67 to 5.09 among white, peach, lavender, and brown lines. Predominant copigments were flavonol glycosides based on kaempferol, quercetin, myricetin, and methylated derivatives. Flavonol aglycones and glycosylation sites differed little among two colour forms of D. gouldii and two D. Jaquelyn Thomas hybrids. Accumulation of quercetin, myricetin, and cyanidin indicated flavonoid 3' and 3',5' hydroxylation activities in several Dendrobium. Additional accumulation of isorhamnetin, syringetin, and peonidin indicated active flavonoid 3'- and 3',5'- O-methyltransferase enzymes. Kluwer Academic Publishers, 1997 Dendrobium nationallicence colour breeding nationallicence monocotyledon nationallicence Orchidaceae nationallicence pelargonidin nationallicence pH nationallicence flavonoid nationallicence anthocyanins nationallicence Kuehnle Adelheid New Zealand Institute for Crop & Food Research Limited, Levin Research Centre, Private Bag 4005, Levin, New Zealand aut Lewis David New Zealand Institute for Crop & Food Research Limited, Levin Research Centre, Private Bag 4005, Levin, New Zealand aut Markham Kenneth New Zealand Institute for Industrial Research & Development, P.O. Box 31-310, Lower Hutt, New Zealand aut Mitchell Kevin New Zealand Institute for Industrial Research & Development, P.O. Box 31-310, Lower Hutt, New Zealand aut Davies Kevin New Zealand Institute for Crop & Food Research Limited, Levin Research Centre, Private Bag 4005, Levin, New Zealand aut Jordan Brian New Zealand Institute for Crop & Food Research Limited, Levin Research Centre, Private Bag 4005, Levin, New Zealand aut Euphytica Kluwer Academic Publishers 95/2(1997-05-01), 187-194 0014-2336 95:2<187 1997 95 10681 https://doi.org/10.1023/A:1002945632713 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002945632713 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuehnle Adelheid New Zealand Institute for Crop & Food Research Limited, Levin Research Centre, Private Bag 4005, Levin, New Zealand aut NATIONALLICENCE 700 1- Lewis David New Zealand Institute for Crop & Food Research Limited, Levin Research Centre, Private Bag 4005, Levin, New Zealand aut NATIONALLICENCE 700 1- Markham Kenneth New Zealand Institute for Industrial Research & Development, P.O. Box 31-310, Lower Hutt, New Zealand aut NATIONALLICENCE 700 1- Mitchell Kevin New Zealand Institute for Industrial Research & Development, P.O. Box 31-310, Lower Hutt, New Zealand aut NATIONALLICENCE 700 1- Davies Kevin New Zealand Institute for Crop & Food Research Limited, Levin Research Centre, Private Bag 4005, Levin, New Zealand aut NATIONALLICENCE 700 1- Jordan Brian New Zealand Institute for Crop & Food Research Limited, Levin Research Centre, Private Bag 4005, Levin, New Zealand aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 95/2(1997-05-01), 187-194 0014-2336 95:2<187 1997 95 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer